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Single-Turnover Flashes to Saturate the QA Reduction in a Leaf were Generated by the Light-Emitting Diodes from a Double Modulation Kinetic Chlorophyll Fluorometer

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Photosynthetica

Abstract

The earlier developed double-modulation chlorphyll (Chl) fluorometer was modified for measurements with intact leaves of higher plants. The Chl fluorometer is based on a non-periodic modulation of both actinic and measuring flashes. In addition, continuous orange actinic and far-red radiation were produced by separate arrays of light-emitting diodes (LEDs). Programmable timing of the flashes allows to cover a wide dynamic range from microseconds to minutes. We have demonstrated that the LEDs can produce single-turnover flashes that saturate QA reduction of intact leaves of Glyceria maxima and shoots of Picea abies.

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References

  • Bolhàr-Nordenkampf, H.R., Öquist, G.: Chlorophyll fluorescence as a tool in photosynthesis research.-In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.). Photosynthesis and Production in a Changing Environment: A Field and Laboratory Manual. Pp. 193-206. Chapman and Hall, London-Glasgow-New York-Tokyo-Melbounre-Madras 1993.

    Google Scholar 

  • Evans, J.R., Jakobsen, I., Ögren, E.: Photosynthetic light-response curves. 2. Gradients of light absorption and photosynthetic capacity.-Planta 189: 191-200, 1993.

    Article  CAS  Google Scholar 

  • Field, T.S., Nedbal, L., Ort, D.R.: Nonphotochemical reduction of the plastoquinone pool in sunflower leaves originates from chlororespiration.-Plant Physiol. 116: 1209-1218, 1998.

    Article  Google Scholar 

  • Lavergne, J., Leci, E.: Properties of inactive Photosystem II centers.-Photosynth. Res. 35: 323-343, 1993.

    Article  CAS  Google Scholar 

  • Marek, M.V., Kalina, J., Nauš, J.: Effect of thinning on parameters of photosynthetic characteristics of Norway spruce canopy. II. Seasonal changes of photosynthetic pigment content.-Lesnictví-Forestry 43: 206-214, 1997.

    Google Scholar 

  • Nedbal, L., Trtílek, M.: Diffuse-light dual modulation fluorometer: monitoring of electron transfer reactions in Synechococcus elongatus exposed to intermittent light.-In: Mathis, P. (ed.) Photosynthesis: From Light to the Biosphere. Vol. V. Pp. 813-816. Kluwer Academic Publ., Dordrecht-Boston-London 1995.

    Google Scholar 

  • Nedbal, L., Trtílek, M., Kaftan, D.: Flash fluorescence induction: A novel method to study regulation of photosystem II.-J. Photochem. Photobiol. B 48: 154-157, 1999.

    Google Scholar 

  • Šesták, Z.: Determination of chlorophylls a and b.-In: Šesták, Z., Čatský, J., Jarvis, P.G. (ed.): Plant Photosynthetic Production. Manual of Methods. Pp. 672-701. Dr W. Junk N.V. Publ., The Hague 1971.

    Google Scholar 

  • Trtílek, M., Kramer, D.M., Koblížek, M., Nedbal, L.: Dual-modulation LED kinetic fluorometer.-J. Lumin. 72–74: 597-599, 1997.

    Article  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Ecological Physiology of Forest Trees, Institute of Landscape Ecology, Pořiči 3b, 603 00, Brno, Czech Republic

    O. Urban

  2. P.S. Instruments Ltd., Koláěkova 39, 621 00, Brno, Czech Republic

    M. Trtilek

  3. Department of Organismic & Evolutionary Biology, Harvard University, 16 Divinity Ave, Cambridge, MA, 02138, USA

    T. Feild

  4. NRC Photosynthesis and Global Climate Change, Institute of Microbiology, Academy of Sciences of the Czech Republic, Opatovický mlýn, 379 81, Třeboň, Czech Republic

    L. Nedbal

Authors
  1. O. Urban
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  2. M. Trtilek
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  3. T. Feild
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  4. L. Nedbal
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Urban, O., Trtilek, M., Feild, T. et al. Single-Turnover Flashes to Saturate the QA Reduction in a Leaf were Generated by the Light-Emitting Diodes from a Double Modulation Kinetic Chlorophyll Fluorometer. Photosynthetica 37, 201–207 (1999). https://doi.org/10.1023/A:1007099720226

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  • DOI: https://doi.org/10.1023/A:1007099720226

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