Abstract
Kernel samples of common and dwarf Brazilian cashew nuts were highly contaminated with field and storage fungi in comparison to healthy ones. In general, dwarf cashews were more contaminated than common. A total of 37 fungal species were identified. Aspergillus niger was the dominant species with more colonies being isolated from dwarf kernels. A. flavus was the next most frequently isolated species. Penicillium brevicompactum, and P. glabrum were the most frequently isolated penicillia, with higher contamination recorded from dwarf kernels. Chaetomium globosum was recorded at a high level. Nine species were recorded from cashew kernels for the first time. Multimycotoxin analysis by tlc and hplc were positive for mycotoxins and other secondary metabolites particularly from the infected samples. Hplc was only carried out on dwarf cashews. Aflatoxins were not detected by quantitative high performance thin layer chromatography.
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Paula Pessoa PFA, Leite LAS, Pimentel CRM. Situacao atual e perspectivas da agroindustria do caju. In: Araujo JPP, Silva VV, eds. Cajucultura: Modernas Tecnicas de Producao. Fortaleza: EMBRAPA/CNPAT, 1995: 23–42.
Freire FCO, Cavalcante MJB, Bezerra JL. Deterioracao fungica de Amendoas de cajueiro no Nordeste brasileiro. Agrotropica 1996; 8: 61–64.
Pitt JI, Rocking AD. Significance of fungi in stored products. In: Champ BR, Highley E, Hocking AD, Pitt JI, eds. Fungi and Mycotoxins in Stored Products. Canberra: ACIAR Proc. No. 36, 1991: 16–21.
Wheeler KA, Miscamble BF, Ng M, Rocking AD, Bhudhasamai K. Survey of mycotoxins associated with maize and other commodities in Thailand. In: Champ BR, Highley E, Hocking AD, Pitt JI, eds. Fungi and Mycotoxins in Stored Products. Canberra: ACIAR Proc. No. 36, 1991: 214–216.
Pitt, JI, Rocking AD, Bhudhasamai K, Miscamble BF, Wheeler KA, Tamboon-Ek P. The normal mycoflora of commodities from Thailand. 1. Nuts and oilseeds. Int J Food Microbiol. 1993; 20: 211–226.
Olunloyo AO. The relation of sugary exudate and insects to fungal infection of developing cashew nuts (Anarcardium occidentale L.) in the plantation. Plant Dis Reptr 1978; 62: 416–419.
Wehner FC, Rabie CJ. The micro-organisms in nuts and dried fruits. Phytophylactia 1970; 2: 165–170.
Weidenborner M, Kunz B. Contamination of different muesli components by fungi. Mycol Res 1994; 98: 582–586.
Miller JD. Mycotoxins. In: Cardwell KF, ed. Proceedings of the Workshop on Mycotoxins in Food in Africa. Cotonou, Benin: IITA. 1996: 18–22.
Mannon J, Johnson E. Fungi down on the farm. New Scient 1985; 28: 12–16.
Bhat RV, Miller JD. Mycotoxins and food supply. Food Nutr Agric (FAO) 1991; 1: 27–31.
Dawson RJ. A global view of the mycotoxin problem. In: Champ BR, Highley E, Hocking AD, Pitt JI, eds. Fungi and Mycotoxins in Stored Products. Canberra: ACIAR Proc. No. 36, 1991: 22–28.
Paterson RRM, Bridge PD. Biochemical techniques for filamentous fungi. Wallingford: CABI International IMI Tech Handbk 1994, No. 1: 1–125.
Frisvad JC, Thrane U. Liquid chromatography of mycotoxins. In: Betina V. ed. Chromatography of Mycotoxins. J Chromat Library 1993, V 54: 253–372.
Gorst-Allman CP, Steyn PS. Screening methods for the detection of thirteen common mycotoxins. J Chromat 1979; 175: 325–331.
Singh K, Frisvad JC, Thrane U, Mathur SB. An illustrated manual on identification of some seed borne aspergilli, fusaria, penicillia and their mycotoxins. Hellerup, Denmark: DGISP, 1991: 133.
Bradburn N, Coker RD, Jewers K. A comparative study of phenyl bonded-phase, CB and Romer clean-up procedures for determining aflatoxin in maize by bidirectional HPTLC. Chromatographia 1990; 29: 177–181.
Cole RJ, Cox RH. Handbook of Toxic Fungal Metabolites. New York: Academic Press, 1991: 937.
Lussenhop J, Wicklow DT. Nitidulid beetles (Nitidulidae: Coleoptera) as vectors of Aspergillus flavus in pre-harvest maize. Trans Mycol Soc Japan 1990; 31: 63–74.
Klich MA, Thomas SH, Mellon JE. Field studies on the mode of entry of Aspergillus flavus into cotton seeds. Mycologia 1984; 76: 665–669.
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Freire, F.C.O., Kozakiewicz, Z., Russell, R. et al. Mycoflora and mycotoxins of Brazilian cashew kernels. Mycopathologia 145, 95–103 (1999). https://doi.org/10.1023/A:1007081319685
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DOI: https://doi.org/10.1023/A:1007081319685