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High temperature conjugation of proteins with carbohydrates

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Abstract

A new procedure was used to conjugate lactose and dextran with BSA without using coupling or activating reagents. The method is simple, rapid and cheap. Reducing sugars covalently bind to proteins when lyophilized together and briefly heated to a high temperature.

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Authors and Affiliations

  1. Department of Tumor Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, 12, Rudolf Weigl, 53-114, Poland

    Janusz Boratyński

  2. Chemistry Department, Ottawa University, K1N 6N5, Canada

    Rene Roy

Authors
  1. Janusz Boratyński
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  2. Rene Roy
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Boratyński, J., Roy, R. High temperature conjugation of proteins with carbohydrates. Glycoconj J 15, 131–138 (1998). https://doi.org/10.1023/A:1007067513242

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  • DOI: https://doi.org/10.1023/A:1007067513242

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