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An Mn2+-stimulated neutral-sphingomyelinase in seminiferous tubules of immature Wistar rats

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Abstract

Mammalian sphingomyelinases have been implicated in many important physiological and pathophysiological processes. The seminiferous tubules of immature (19 day-old) Wistar rats have at least three types of sphingomyelinases, a lysosomal one and two microsomal ones. One of the microsomal sphingomyelinases is active at pH 6.5 and is stimulated by Mn2+ > Co2+ > Mg2+, and the other is active at pH 7.4 and is stimulated by Mn2+ > Mg2+ and inhibited by Co2+. The two microsomal enzymes are only slightly inhibited by EDTA and at pH 7.4 the stimulatory effects of Mn2+ and Mg2+ are additive. These data characterize the existence of two different membrane-bound sphingomyelinases in the seminiferous tubules of the rat.

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Authors and Affiliations

  1. Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil

    Paulo Eduardo Raimann, Izabel Cristina Custodio de Souza, Elena Aida Bernard & Fátima Costa Rodrigues Guma

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  1. Paulo Eduardo Raimann
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  2. Izabel Cristina Custodio de Souza
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  3. Elena Aida Bernard
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  4. Fátima Costa Rodrigues Guma
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Raimann, P.E., Custodio de Souza, I.C., Bernard, E.A. et al. An Mn2+-stimulated neutral-sphingomyelinase in seminiferous tubules of immature Wistar rats. Mol Cell Biochem 201, 125–129 (1999). https://doi.org/10.1023/A:1007046024246

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