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Antioxidant properties of (-)-epicatechin-3-gallate and its inhibition of Cr (VI)-induced DNA damage and Cr (IV)- or TPA-stimulated NF-κB activation

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Abstract

Electron spin resonance (ESR) spin trapping was utilized to investigate the scavenging effects on hydroxyl radicals (·OH) and superoxide radicals (O2·-) by (-)-epigallocatechin-3-gallate (EGCG), one of the major anticancer compounds in tea. The spin trap used was 5,5-dimethyl-pyrroline N-oxide (DMPO). The Fenton reaction (Fe2+ + H2O2→ Fe3+ +·OH + OH-) was used as a source of ·OH radicals. EGCG efficiently scavenges ·OH radicals with reaction rate of 4.62 × 1011 M- 1sec- 1, which is an order of magnitude higher than several well recognized antioxidants, such as ascorbate, glutathione and cysteine. It also scavenges O2·- radicals as demonstrated by using xanthine and xanthine oxidase system as a source of O2·- radicals. Through its antioxidant properties, EGCG exhibited a protective effect against DNA damage induced by Cr(VI). EGCG also inhibited activation of nuclear transcription factor NF-κB induced by Cr(IV) and 12-o-tetradecanoylphorbol-13-acetate (TPA). The present studies provide a mechanistic basis for the reported anticarcinogenic properties of EGCG and related tea products.

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Authors and Affiliations

  1. Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, USA

    Xianglin Shi, Jianping Ye, Stephen Leonard, Min Ding, Val Vallyathan & Vincent Castranova

  2. Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, USA

    Yon Rojanasakul

  3. The Hormel Institute, University of Minnesota, Austin, USA

    Zigang Dong

Authors
  1. Xianglin Shi
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  2. Jianping Ye
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  3. Stephen Leonard
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  4. Min Ding
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  5. Val Vallyathan
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  6. Vincent Castranova
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  7. Yon Rojanasakul
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  8. Zigang Dong
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Shi, X., Ye, J., Leonard, S. et al. Antioxidant properties of (-)-epicatechin-3-gallate and its inhibition of Cr (VI)-induced DNA damage and Cr (IV)- or TPA-stimulated NF-κB activation. Mol Cell Biochem 206, 125–132 (2000). https://doi.org/10.1023/A:1007012403691

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  • DOI: https://doi.org/10.1023/A:1007012403691

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