Abstract
Trichophyton tonsurans is an anthropophilic dermatophyte mostly causing tinea capitis and tinea corporis. This study was carried out to identify T. tonsurans and to clarify whether it has any genetic differences depending on the phenotype or region of isolation by random amplified polymorphic DNA (RAPD) analysis with three random primers. The assay was performed in 11 Korean, 2 Japanese, 2 Taiwanese, 5 Brazilian and 1 American isolates of T. tonsurans together with the other 10 anamorphic species of dermatophytes and 3 Arthroderma spp. All tested species of dermatophytes showed distinct bands and T. tonsurans was differentiated from other dermatophytes. It was most clearly ifferentiated from T. mentagrophytes by using primer 5‘-GAAGGCTCCC-3’ (OPAO-15). No difference was found in RAPD band patterns in all strains of T. tonsurans with these random primers. It was considered that T. tonsurans is a genetically homogeneous species regardless of its isolation regions, morphologic or physiologic characteristics.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kwon-Chung KJ, Bennett JE. Medical Mycology. Philadelphia: Lea and Febiger, 1992: 148.
Rippon JW. Medical Mycology, 3rd ed. Philadelphia: W. B. Saunders Co., 1988: 257–259.
Kane J, Summerbell R, Sigler L, Krajden S, Land G. Laboratory Handbook of Dermatophytes. Belmont: Star Publishing Co., 1997: 174–177.
Babel DE, Rogers AL, Beneke ES. Dermatophytosis of scalp: Incidence, immune response, and epidemiology. Mycopathologia 1990; 109: 69–73.
Frieden IJ, Howard R. Tinea capitis: Epidemiology, diagnosis, treatment, and control. J Am Acad Dermatol 1994; 31: S42–S46.
Aly R. Ecology and epidemiology of dermatophyte infections. J Am Acad Dermatol 1994; 31: S21–S25.
Chao SC, Hsu MM. Trichophyton tonsurans infection in Tainan area. J Formosan Med Assoc 1994; 98: 697–701.
Takahashi S. 1992 Epidemiological survey of dermatomycoses in Japan. Jpn J Med Mycol 1995; 36: 87–95.
Suh SB. Dermatophytosis and its causative agents in Korea. Kor J Med Mycol 1996; 1: 1–10.
Gan Y, Liu B, Song Y et al. Geographical distribution of species of etiologic fungi of dermatophytosis in northeast China. Jpn J Med Mycol 1997; 38: 9–12.
Jun JB, Kim YD, Lee JW et al. An epidemiological and mycological study on trichophytosis gladiatorum prevailing among Korean wrestlers. 13th Congress of the International Society for Human and Animal Mycology, Parma, Italia, 1997; O38 (Abstract).
Foster LM, Kozak KR, Loftus MG et al. The polymerase chain reaction and its application to filamentous fungi. Mycol Res 1993; 97: 769–781.
Mochizuchi T, Sugie N, Uehara M. Random amplification of polymorphic DNA is useful for the differentiation of several anthropophilic dermatophytes. Mycoses 1997; 40: 405–409.
Mochizuchi T, Uehara M, Menon T, Ranganathan S. Minipreparation of total cellular DNA is useful as an alternative molecular marker of mitochondrial DNA for the identification of Trichophyton mentagrophytes and T. rubrum. Mycoses 1996; 39: 31–35.
Makimura K, Goto H, Mochizuki T, Hasegawa A, Uchida K, Yamaguchi H. Molecular biological approach to phylogeny and identification of dermatophytes. Jpn J Med Mycol 1997; 38: 5–8.
Mochizuchi T, Takada K, Watanabe S, Kawasaki M, Ishizaki H. Taxonomy of Trichophyton interdigitale (Trichophyton mentagrophytes var. interdigitale) by restriction enzyme analysis of mitochondrial DNA. J Med Vet Mycol 1990; 28: 191–196.
Liu D, Coloe S, Baird R, Pederson J. PCR identification of Trichophyton mentagrohytes var. interdigitale and T. mentagrophytes var. mentagrophytes dermatophytes with a random primer. J Med Microbiol 1997; 46: 1043–1046.
Nishio K, Kawasaki M, Ishizaki H. Phylogeny of the genera Trichophyton using mitochondrial DNA analysis. Mycopathologia 1992; 117: 127–132.
Ishizaki H. Fungal taxonomy based on mitochondrial DNA analysis. Jpn J Med Mycol 1993; 34(3): 243–251.
Makimura K, ed. Molecular Diagnosis of Fungal Infection, Protocols in Pathogenic Fungi Genome Analysis (in Japanese). Tokyo: Medical Sense Co., 1997: 70–75.
Liu D, Coloe S, Baird R, Pedersen J. Molecular determination of dermatophyte fungi using the arbitrarily primed polymerase chain reaction. Brit J Dermatol 1997; 137: 351–355.
Makimura K, Murayama SY, Yamaguchi H. Detection of wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 1994; 40: 358–364.
Poonwan N, Mikami Y, Poosuwan S, Boon-Long J, Mekha N, Kusum M, Yazawa K, Tanaka R, Nishimura K, Konyama K. Serotyping of Cryptococcus neoformans strains isolated from clinical specimens in Thailand and their susceptibility to various antifungal agents. Eur J Epidemiol 1997; 13: 335–340.
Liu D, Coloe S, Pederson J, Baird R. Use of arbitrarily primed polymerase chain reaction to differentiate Trichophyton dermatophytes. FEMS Microbiol Lett 1996; 136: 147–150.
Zhong Z, Li R, Li D, Wang D. Typing of common dermatophytes by random amplification of polymorphic DNA. Jpn J Med Mycol 1997; 38: 239–246.
Gräser Y, Fari M, Presber W, Sterry W, Tietz H-J. Identification of common dermatophytes (Trichophyton, Microsporum, Epidermophyton) using polymerase chain reaction. Brit J Dermatol 1998; 138: 576–582.
Rights and permissions
About this article
Cite this article
Kim, J.A., Takizawa, K., Fukushima, K. et al. Identification and genetic homogeneity of Trichophyton tonsurans isolated from several regions by random amplified polymorphic DNA. Mycopathologia 145, 1–6 (1999). https://doi.org/10.1023/A:1007008401122
Issue Date:
DOI: https://doi.org/10.1023/A:1007008401122