Abstract
Transmannosylations catalysed by β-mannosidase from snail viscera or β-galactosidase from Aspergillus oryzae were accomplished with 4-nitrophenyl β D -mannopyranoside as donor substrate. With suitable hydrophobic acceptor molecules preferentially β1-4-linked disaccharides were obtained. The activities of both glycosidases in buffer cosolvent mixtures were determined, and conditions for their immobilization were elaborated and optimized. A model of the enzymic transfer mechanism is suggested.
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Taubken, N., Thiem, J. Hydrolase-catalyzed β-mannosylations. Glycoconj J 15, 757–767 (1998). https://doi.org/10.1023/A:1006999614526
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DOI: https://doi.org/10.1023/A:1006999614526