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Hepatic production of apolar aldehydes in rats with carbon tetrachloride-induced cirrhosis

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Abstract

The aim of this study was to identify apolar aldehydes in liver homogenates from rats with CCl4-induced cirrhosis and, as a corollary, the antioxidant effect of zinc administration. The study was performed in five control rats and in ten cirrhotic rats which were further sub-divided into two groups to receive either a standard diet or one supplemented with zinc. The percentage of hepatic fibrosis, plasma malondialdehyde concentration and alanine aminotransferase activity were measured as well as the following aldehydes: hexanal, octanal, decanal, 2-hexenal, 2-octenal, 2-nonenal, 2,4-heptadienal and 2,4-decadienal. Of the 10 cirrhotic rats, 4 had elevated concentrations of the highly toxic 2,4-dialkenals which coincided with a higher percentage of fibrosis and plasma alanine aminotransferase activity. These aldehydes were not observed in the control group. Zinc administration was associated with a reduction of the hepatic malondialdehyde concentration and an amelioration on the degree of hepatic injury. In conclusion, this study demonstrates the presence of the highly toxic 2,4-dialkenals in hepatic tissue of rats whith CCl4-induced cirrhosis. Results obtained would suggest that these particular aldehydes may be related to the severity of the hepatic injury.

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Authors and Affiliations

  1. Unitat Clínico-Experimental d'Investigació, Hospital Universitari de Sant Joan-Facultat de Medicina, Universitat Rovira i Virgili, Reus, Catalunya, Spain

    Natàliae Ferré, Josefa Girona, Maria Cabré, Jorge Joven, Agnes La Ville, Lluis Masana, José Luis Patern´in & Jordi Camps

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  1. Natàliae Ferré
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  2. Josefa Girona
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  3. Maria Cabré
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  4. Jorge Joven
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  5. Agnes La Ville
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  6. Lluis Masana
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  7. José Luis Patern´in
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  8. Jordi Camps
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Ferré, N., Girona, J., Cabré, M. et al. Hepatic production of apolar aldehydes in rats with carbon tetrachloride-induced cirrhosis. Mol Cell Biochem 198, 57–60 (1999). https://doi.org/10.1023/A:1006998028528

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