Skip to main content
Log in

Characterization of Photosynthetic Apparatus of Pea Chlorophyll Mutants and their Heterotic F1 Hybrids with Standard Genotype (cv. Torsdag)

  • Published:
Photosynthetica

Abstract

In comparison to cv. Torsdag, in leaves of low-productive Pisum sativum L. chlorophyll mutants the decrease in chlorophyll content was caused by the decrease in cell number per unit volume. Qualitative changes in activities of photosystem (PS) 1 in mutant M2004, and quantitative changes of PS1 and PS2 in mutants M2004 and M2014 and in hybrids were also found. However, the activity of ribulose-1,5-bisphosphate carboxylase (RuBPC) in M2014, and those of RuBPC and glyceraldehyde phosphate dehydrogenase in M2004 and hybrids were higher than in cv. Torsdag. The hybrids inherited the normal structure of photosynthetic apparatus of standard genotype in parallel with the compensatory gene complex of M2004, which was expressed at many levels of organization. This may be the basis of hybrid vigour in this case.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    Google Scholar 

  • Butterfass, T.: Control of plastid division by means of nuclear DNA amount.-Protoplasma 76: 167–195, 1973.

    Google Scholar 

  • Chetverikov, A.G.: [Use of EPR-spectroscopy for studying physiology of photosynthesis.].-Fiziol. Rast. 30: 682–692, 1983. [In Russ.]

    Google Scholar 

  • Edwards, I., Walker, D.: Fotosintez C3-C4-Rasteniï: Mekhanizmy i Regulyatsiya. [Photosynthesis of C3 and C4-Plants: Mechanisms and Regulation.]-Mir, Moskva 1986. [In Russ.]

    Google Scholar 

  • Ferte, N., Jacquot, J.-P., Meunier, J.-C.: Structural immunological and kinetic comparison of NADP-dependent malate dehydrogenases from spinach (C3) and corn (C4) chloroplasts.-Eur. J. Biochem. 154: 587–595, 1986.

    Google Scholar 

  • Govindjee (ed.): Photosynthesis. Vol. I. Energy Conversion by Plants and Bacteria.-Academic Press, New York-London-Paris-San Diego-San Francisco-São Paulo-Sydney-Tokyo-Toronto 1982.

    Google Scholar 

  • Kalashnikov, Yu.E., Vaishlya, O.B.: [Oxygen activation and enzymes of protection in pea pigment mutants under aeration and hypoxy.]-Tsitologiya 33: 104, 1991. [In Russ.]

    Google Scholar 

  • Knoetzel, J., Braumann, T., Grimme, L.H.: Pigment-protein complexes of green algae: improved methodological steps for the quantification of pigments in pigment-protein complexes derived from the green algae Chlorella and Chlamydomonas.-J. Photochem. Photobiol. B 1: 475–479, 1988.

    Google Scholar 

  • Krebs, D., Synková, H., Avratovščuková, N., Kočová, M., Šesták, Z.: Chlorophyll fluorescence measurements for genetic analysis of maize cultivars.-Photosynthetica 32: 595–608, 1996.

    Google Scholar 

  • Ladygin, V.G., Bil', K.Ya.: Chlorophyll form absorbing at 684 nm as antenna of Photosystem 2 in chloroplasts of C4-plant leaves.-Photosynthetica 15: 49–54, 1981.

    Google Scholar 

  • Ladygin, V.G., Chunaev, A.S., Semenova, G.A., Mirnaya, O.N., Kornyushenko, G.A., Maslov, V.G.: [Peculiarities of chloroplast ultrastructure in Chlamydomonas reinhardtii by the interaction of genes controlling carotenoid deficiency and chlorophyll b-lack.].-Tsitologiya 30: 19–25, 1988. [In Russ.]

    Google Scholar 

  • Lance, C., Rustin, P.: The central role of malate in plant metabolism.-Physiol. vég. 22: 625–641, 1984.

    Google Scholar 

  • Lawlor, D.W.: Photosynthesis: Metabolism, Control and Physiology.-Longman, New York 1987.

    Google Scholar 

  • Lichtenthaler, H.K.: Functional organization of carotenoids and prenylquinones in the photosynthetic membrane.-In: Stumpf, P.K., Mudd, J.B. (ed.): The Metabolism, Structure, and Function of Plant Lipids. Pp. 63–79. Plenum Press, New York 1987.

    Google Scholar 

  • Lichtenthaler, H.K., Wellburn, A.R.: Determination of total carotenoids and chlorophyll a and b leaf extracts in different solvents.-Biochem. Soc. Trans. 11: 591–592, 1983.

    Google Scholar 

  • Michel, H., Tellenbach, H., Boschetti, A.: A chlorophyll b-less mutant of Chlamydomonas reinhardii lacking in the light-harvesting chlorophyll a/b-protein complex but not in its apoproteins.-Biochim. biophys. Acta 725: 417–424, 1983.

    Google Scholar 

  • Mokronosov, A.T.: [Mesostructure and functional activity of photosynthetic apparatus.]-In: Mezostruktura i Funktsional'naya Aktivnost' Fotosinteticheskogo Apparata. Pp. 5–30. Ural'skiï Gosudarstvennyï Universitet, Sverdlovsk 1978. [In Russ.]

    Google Scholar 

  • Mullet, J.E.: Chloroplast development and gene expression.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 475–502, 1988.

    Google Scholar 

  • Nishimura, M., Ito, T., Chance, B.: Studies in bacterial photophosphorylation. III. A sensitive and rapid method of determination of photophosphorylation.-Biochim. biophys. Acta 59: 177–182, 1962.

    Google Scholar 

  • Öquist, G., Samuelsson, G., Bishop, N.I.: On the role of β-carotene in the reaction center chlorophyll a antennae of photosystem I.-Physiol. Plant. 50: 63–71, 1980.

    Google Scholar 

  • Perrot-Rechenmann, G., Vidal, J., Brulfert, A.: A comparative immunochemical localization study of phosphoenolpyruvate carboxylase in leaves of higher plants.-Planta 155: 24–31, 1982.

    Google Scholar 

  • Pyke, K.A., Leech, R.M.: The control of chloroplast number in wheat mesophyll cells.-Planta 170: 416–420, 1987.

    Google Scholar 

  • Rathnam, C.K.M., Edwards, G.E.: Intracellular localization of certain photosynthetic enzymes in bundle sheath cells of plants possessing the C4 pathway of photosynthesis.-Arch. Biochem. Biophys. 171: 214–225, 1975.

    Google Scholar 

  • Roshchina, V.V., Karpilova, I.P., Bozhok, G.V., Gostimskiï, S.A.: Photosynthesis of pea mutants with damaged photosystems.-Photosynthetica 17: 590–596, 1983.

    Google Scholar 

  • Rubin, B.A., Gavrilenko, V.F.: Biokhimiya i Fiziologiya Fotosinteza. [Biochemistry and Physiology of Photosynthesis.]-Moskov. Univ., Moskva 1977. [In Russ.]

    Google Scholar 

  • Scheibe, R., Beck, E.: Formation of C4-dicarboxylic acids by intact spinach chloroplasts.-Planta 125: 63–67, 1975.

    Google Scholar 

  • Schwarz, H.P.: The light harvesting chlorophyll protein complex from wild type and chlorophyll deficient mutants of pea (Pisum sativum L.).-In: Workshop Mol. Struct. Funct. Light-Harvest. Pigment-Protein Complexes and Photosynth. React. Cent. Pp. 113–114. Zurich 1983.

  • Semenova, G.A.: [Electron-dense substance in mesophyll cells of potato leaves.]-Fiziol. Rast. 32: 461–464, 1985. [In Russ.]

    Google Scholar 

  • Shumnyï, V.K., Sokolov, V.A., Vershinin, A.V.: [Heterosis and mechanisms of superdominancy.]-In: Geterosis. Pp. 109–141. Nauka Tekhnika, Minsk 1982. [In Russ.]

    Google Scholar 

  • Singh, P., Naik, M.S.: Effect of photosynthesis on dark mitochondrial respiration in green cells.-FEBS Lett. 165: 145–150, 1984.

    Google Scholar 

  • Sokolov, V.A.: [Localization of mutant gene controlled pigmentation type chlorotica in pea.]-Dokl. Akad. Nauk SSSR 308: 1244–1246, 1989. [In Russ.]

    Google Scholar 

  • Sokolov, V.A., Shumnyï, V.K., Gorbunova, G.A., Sushkova, O.A.: [Comparative study of glucose-6-phosphate dehydrogenase in pea lines ontogeny in connection with heterosis.]-Genetika 19: 2069–2072, 1983. [In Russ.]

    Google Scholar 

  • Sokolov, V.A., Shumnyi, V.K., Tsonev, Ts., Stanev, V., Danailov, G., Dobrinova, K.: [Frequency, size and functional characteristics of stomata as regards pea heterosis.]-Izv. Akad. Nauk SSSR, Ser. biol. 1988(2): 89–94, 1988. [In Russ.]

    Google Scholar 

  • Stitt, M., Quick, W.P.: Photosynthetic carbon partitioning: its regulation and possibilities for manipulation.-Physiol. Plant. 77: 633–641, 1989.

    Google Scholar 

  • Tsel'niker, Yu.L.: Fiziologicheskie Osnovy Tenevynoslivosti Drevesnykh Rasteniï. [Physiological Bases of Shade Tolerance in Woody Plants.]-Nauka, Moskva 1978. [In Russ.]

    Google Scholar 

  • Vaishlya, O.B.: [Physiological aspects of monohybrid heterosis of pea.]-In: Izogennye Linii i Geneticheskie Kollektsii. Pp. 146–150. Novosibirsk 1993. [In Russ.]

  • Vayshla, O.B.: The physiological study of pea heterosis.-In: Vth International Youth Symposium on Plant Metabolism Regulation. Abstracts. P. 17. Varna 1990.

  • Vayshla, O.B.: Regulation of malatedehydrogenase system activity by the level of endogenic phytohormones in pea chlorophyll mutants leaves.-In: Physical-chemical Basis of Plant Physiology. Abstracts. Pp. 59–60. Pushchino 1996.

  • Woodrow, I.E., Berry, J.A.: Enzymatic regulation of photosynthesis CO2 fixation in C3-plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 533–594, 1988.

    Google Scholar 

  • Zabotin, A.I.: [Determination of photoinduced changes in pH during study of photophosphorylation.]-In: Metody Issledovania Fotofosforilirovaniya. Pp. 182–195. Pushchino 1970. [In Russ.]

  • Zalenskiï, O.V., Glagoleva, T.A., Chulanovskaya, M.V.: [Influence of inhibitors of photophosphorylation on photosynthesis and metabolism of assimilating cells of Chlorella pyrenoidosa Chick.]-Bot. Zh. 51: 1718–1721, 1966. [In Russ.]

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vaishlya, O., Ladygin, V., Sokolov, V. et al. Characterization of Photosynthetic Apparatus of Pea Chlorophyll Mutants and their Heterotic F1 Hybrids with Standard Genotype (cv. Torsdag). Photosynthetica 35, 429–443 (1998). https://doi.org/10.1023/A:1006972504239

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006972504239

Navigation