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Inhibition of phagocytic activity by the N-acetyl-D-galactosamine-specific lectin from Amaranthus leucocarpus

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Abstract

Amaranthus leucocarpus lectin (ALL), specific for N-acetyl-D-galactosamine, induces inhibition of the erythrophagocytic activity of resident murine peritoneal macrophages and of the macrophage-like cell line J-774. This effect was observed only in macrophages that were Mac-2 (CD11c/CD18 or CR4) negative, indicating that macrophage activation induces important modification to the glycosylation (mainly O-glycosylation) of the membrane. Receptors for IgM and C3b remain unaltered after lectin treatment. Ultrastructural analysis revealed (a) that ALL induced the formation of pinocytic vacuoles, and (b) a regular distribution over the macrophage membrane as well as endosomal vesicles of the gold labeled ALL. Our results suggest that macrophage membrane glycoproteins with constitutive N-acetyl-D-galactosamine residues participate in the regulation of pinocytic-phagocytic vacuole formation.

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Authors and Affiliations

  1. Laboratorio de Inmunologia, Departamento de Bioquimica, Facultad de Medicina UNAM, P.O. BOX 70159, 04510, Mexico

    Guadalupe Maldonado, Concepcion Agundis & Edgar Zenteno

  2. Departmento de Bioquimica, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, 14080, Mexico

    Patricia Gorocica

  3. Departmento de Biologia Celular y Tisular, Facultad de Medicina UNAM, 04510, Mexico

    Armando Perez

  4. Departmento de Farmacologia, Facultad de Medicina UNAM, 04510, Mexico

    Juan Molina

Authors
  1. Guadalupe Maldonado
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  2. Patricia Gorocica
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  3. Concepcion Agundis
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  4. Armando Perez
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  5. Juan Molina
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  6. Edgar Zenteno
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Maldonado, G., Gorocica, P., Agundis, C. et al. Inhibition of phagocytic activity by the N-acetyl-D-galactosamine-specific lectin from Amaranthus leucocarpus. Glycoconj J 15, 615–622 (1998). https://doi.org/10.1023/A:1006972011711

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  • DOI: https://doi.org/10.1023/A:1006972011711

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