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Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling

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Abstract

To identify Mycobacterium bovis glycoproteins, carbohydrates present in a delipidized M. bovis culture filtrate protein extract were biotin-hydrazide labeled. 11 carbohydrate-containing protein with a moleculra weight of 15-, 19-, 25-, 32-, 35-, 39-, 42-, 48-, 52-, 58-, and 62-kDa were detected. The 52- and 32-kDa protein were deglycosylated by endoglycosidase-F.

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Authors and Affiliations

  1. Depto. Biología Celular, Instituto Nal. Cardiología, Ignacio Chávez, México, 14080 DF

    Felipe Massó, Elvira Varela, Araceli Páez, Edgar Zenteno & Luis Felipe Monta$ntilde;o

Authors
  1. Felipe Massó
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  2. Elvira Varela
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  3. Araceli Páez
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  4. Edgar Zenteno
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  5. Luis Felipe Monta$ntilde;o
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Massó, F., Varela, E., Páez, A. et al. Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling. Glycoconj J 15, 843–846 (1998). https://doi.org/10.1023/A:1006963116919

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  • DOI: https://doi.org/10.1023/A:1006963116919

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