Abstract
V79 fibroblasts were treated with ultraviolet (UV) C radiation alone as well as in conjunction with chronic oxidative stress. The effects of these treatments on calcium signaling were observed at 30 min post-irradiation. In the absence of extracellular calcium, thrombin released calcium from internal stores of UVC-irradiated V79 fibroblasts even after exposure to neomycin. In neomycin-treated control and chronic oxidative stress cells, no calcium release by thrombin was observed after chelation of external calcium. Calcium release by thrombin from internal stores of UV-irradiated and neomycin-treated cells was completely abolished by pretreatment with N-acetyl cysteine and dexamethasone. Cellular total soluble thiol content which is a good indicator of cellular reduced glutathione (GSH) level was significantly elevated 30 min after ultraviolet radiation, indicating an adaptive response after oxidative stress. Chronic oxidative stress alone resulted in a much smaller increase in GSH but chronic oxidative stress in conjunction with UVC produced a very prominent elevation in GSH levels. Our data suggest that thrombin can cause calcium release from internal stores of ultraviolet-irradiated fibroblasts which is independent of phosphatidylinositol bisphosphate hydrolysis and is directly related to the level of oxidative stress. Involvement of phopholipase A2 and a role for its products as possible mediators of calcium release from intracellular stores, is strongly indicated.
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Bagchi, S., Bhaumik, G. & Raha, S. Thrombin releases calcium from internal stores of ultraviolet C-treated V79 fibroblasts independent of phosphatidymositol bisphosphate hydrolysis: Role of oxidative stress. Mol Cell Biochem 196, 23–30 (1999). https://doi.org/10.1023/A:1006957926978
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DOI: https://doi.org/10.1023/A:1006957926978