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A novel method for determination of α1,6fucosyltransferase activity using a reducing oligosaccharide from egg yolk as a specific acceptor

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Abstract

A new method for determination of α1,6fucosyltransferase activity has been described. Recently, the disialyl-biantennary undecasaccharide was prepared in high yield from egg yolk [(1996), Carbohydr Lett 2: 137–42]. By treatment of this oligosaccharide with neuraminidase and β-galactosidase, we readily obtained an asialo-agalacto-biantennary heptasaccharide (GlcNAcβ 1,2Manα1,6[GlcNAcβ1,2Manα1,3]Manβ1,4GlcNAcβ1,4GlcNAc). Using this asialo-agalacto-oligosaccharide as an acceptor, fucosyltransferases from human plasma and extracts of various human hepatoma cell lines were assayed in the presence of GDP-[3H]fucose. The reaction mixture was applied to a column of GlcNAc-binding, Psathyrella velutina lectin coupled gel. All the fucosylated acceptor were bound to the column which was eluted with 50 mM GlcNAc. Structural analyses revealed that only the innermost GlcNAc residue of the acceptor was fucosylated through an α1,6-linkage, and the oligosaccharide prepared could be used as a specific acceptor for α1,6fucosyltransferase. The present method was used to screen plasma α1,6fucosyltransferase in several patient groups, and significantly elevated activities were found in samples from patients with liver diseases, including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma.

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References

  1. Salmon C, Cartron JP, Rouger P (1984) In Human Blood Groups, pp. 54–80. New York: Masson Publishing.

    Google Scholar 

  2. Hakomori S (1996) Cancer Res 56: 5309–18.

    Google Scholar 

  3. Schachter H (1995) In Glycoproteins Montreuil J, Vliegenth art JFG, Schachter H, eds), pp. 123–126. Amsterdam: Elsevier.

    Google Scholar 

  4. Madiyalakan R, Yazawa S, Abbas SA, Barlow JJ, Matta KL (1986) Anal Biochem 152: 22–8.

    Google Scholar 

  5. Yazawa S, Madiyalakan R, Jain RK, Shimoda N, Matta KL (1990) Anal Biochem 187: 374–8.

    Google Scholar 

  6. Tachikawa T, Yazawa S, Asao T, Shin S, Yanaihara N (1991) Clin Chem 37: 2081–6.

    Google Scholar 

  7. Yazawa S, Takeya A, Hosomi O, Nakajima T, Shimoda N, Ohi H, Tachikawa T, Piscorz CF, Jain RK, Matta KL (1992) Clin Chem 38: 2392–5.

    Google Scholar 

  8. Yazawa S, Nakajima T, Kameyama N, Saga K, Tachikawa T (1993) Carbohydr Res 239: 329–35.

    Google Scholar 

  9. Chester MA, Yates AD, Watkins WM (1976) Eur J Biochem 69: 583–92.

    Google Scholar 

  10. DiCioccio RA, Barlow JJ, Matta KL (1980) Clin Chem Acta 108: 41–8.

    Google Scholar 

  11. Schachter H, Narashimhan S, Gleeson P, Vella G (1983) In Methods in Enzymology (Fleischer S and Fleischer B., eds), vol. 98, pp 98–110. New York: Academic Press.

    Google Scholar 

  12. Voynow JA, Scanlin TF, Glick MC (1988) Anal Biochem 168: 367–73.

    Google Scholar 

  13. Uozumi N, Teshima T, Yamamoto T, Nishikawa A, Gao YE, Miyoshi E, Gao CX, Noda K, Islam KN, Ihara Y, Fujii S Shiba T, Taniguchi N (1996) J Biochem 120: 385–92.

    Google Scholar 

  14. Yamashita K, Taketa K, Nishi S, Fukushima K, Ohkura T (1993) Cancer Res 53: 2970–5.

    Google Scholar 

  15. Aoyagi Y, Suzuki Y, Isemura M, Nomoto M, Sekine C, Igarashi K, Ichida F (1988) Cancer 61: 769–74.

    Google Scholar 

  16. Hutchinson WL, Du MQ, Johnson PJ, Williams R (1991) Hepatology 13: 683–8.

    Google Scholar 

  17. Kochibe N, Furukawa K (1980) Biochemistry 19: 2841–6.

    Google Scholar 

  18. Kochibe N (1973) J Biochem 74: 1141–6.

    Google Scholar 

  19. Nakabayashi H, Taketa K, Miyamoto K, Yamamoto T, Sato J (1982) Cancer Res 42: 3858–63.

    Google Scholar 

  20. Enoki Y, Koketsu M, Juneja LR, Kim M, Yamamoto T (1996) Carbohydr Lett 2: 137–42.

    Google Scholar 

  21. Kochibe N, Matta KL (1989) J Biol Chem 264: 173–7.

    Google Scholar 

  22. Endo T, Ohbayashi H, Kanagawa K, Kochibe N, Kobata A (1992) J Biol Chem 267: 707–13.

    Google Scholar 

  23. Yazawa S, Furukawa K, Kochibe N (1984) J Biochem 96: 1737–42.

    Google Scholar 

  24. Yazawa S, Kochibe N, Asao T (1980) Immunol Invest 19: 319–27.

    Google Scholar 

  25. Yamashita K, Kochibe N, Ohkura T, Ueda I, Kobata A (1985) J Biol Chem 260: 4688–93.

    Google Scholar 

  26. Kondo A, Suzuki J, Kuraya N, Hase S, Kato I, Ikenaka T (1990) Agr Biol Chem 54: 2169–70.

    Google Scholar 

  27. Bosman HB, Hagopian A, Eylar EH (1968) Arch Biochem Biophys 128: 470–81.

    Google Scholar 

  28. Wilson JR, Williams D, Schachter H (1976) Biochem Biophys Res Commun 72: 909–16.

    Google Scholar 

  29. Longmore GD, Schachter H (1982) Carbohyr Res 100: 365–92.

    Google Scholar 

  30. Voynow JA, Scanlin TF, Glick MC (1988) Anal Biochem 168: 367–73.

    Google Scholar 

  31. Voynow JA, Kaiger RS, Scanlin TF, Glick MC (1991) J Biol Chem 266: 21572–7.

    Google Scholar 

  32. Kosielak J, Pacuszka T, Kubin J, Zdziechows H(1987) Glycoconjugate J 4: 43–9.

    Google Scholar 

  33. Yazawa S, Nakamura T, Asao T, Nagamachi Y, Sagi M, Matta KL, Tachikawa T, Akamastu S (1993) Jpn J Cancer Res 84: 989–95.

    Google Scholar 

  34. Yazawa S, Madiyalakan R, Izawa H, Asao T, Furukawa T, Matta KL (1988) Cancer 62: 516–20.

    Google Scholar 

  35. Yazawa S, Asao T, Nagamachi Y, Abbas SA, Matta KL (1989) J Cancer Res Clin Oncol 115: 451–5.

    Google Scholar 

  36. Asao T, Yazawa S, Nagamachi, Abbas SA, Matta KL (1989) Cancer 64: 2541–5.

    Google Scholar 

  37. Yazawa S, Asao T, Nagamachi Y (1989) J Tumor Marker Oncol 4: 355–62.

    Google Scholar 

  38. Yazawa S, Nishihara S, Iwasaki H, Asao T, Nagamachi Y, Matta KL, Narimatsu H (1995) Cancer Res 55: 1473–8.

    Google Scholar 

  39. Hada T, Fukui K, Ohno M, Akamatsu S, Yazawa S, Enomoto K, Yamaguchi K, Matsuda Y, Amuro Y, Yamanaka N, Okamoto E, Higashi K (1995) Glycoconjugate J 12: 627–31.

    Google Scholar 

  40. Akamatsu S, Yazawa S, Zenita K, Matsumoto H, Tachikawa T, Kannagi R (1996) Glycoconjugate J 13: 1021–9.

    Google Scholar 

  41. Nakamura J, Mogi A, Asao T, Nagamachi Y, Yazawa S (1997) Anticancer Res 17: 4563–70.

    Google Scholar 

  42. Koketsu M, Juneja LR, Kim M, Ohta M, Matsuura F, Yamamoto T (1993) J Food Sci 58: 743–7.

    Google Scholar 

  43. Seko A, Koketsu M, Nishizono M, Enoki Y, Ibrahim H, Juneja LR, Kim M, Yamamoto T (1997) Biochim Biophys Acta 1355: 23–32.

    Google Scholar 

  44. Kornfeld K, Reitman ML, Kornfeld R (1981) J Biol Chem 256: 6633–40.

    Google Scholar 

  45. Kessel D, Chou T (1979) Biochem J 181: 767–69.

    Google Scholar 

  46. Holmes EH, Xu Z, Sherwood AL, Macher BA (1995) J Biol Chem 270: 8145–51.

    Google Scholar 

  47. Clarke JL, Watkins WM (1996) J Biol Chem 271: 10317–28.

    Google Scholar 

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Authors and Affiliations

  1. Japan Immunresearch Laboratories, Takasaki, 370, Japan

    Shin Yazawa, Toyo Nishimura, Chikako Shima, Izumi Takai & Masakazu Adachi

  2. Dept. Biology, Faculty of Education, Gunma University, Maebashi, 371, Japan

    Naohisa Kochibe

  3. First Department of Surgery, Gunma University School of Medicine, Maebashi, 371, Japan

    Takayuki Asao

  4. Third Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, 663, Japan

    Toshikazu Hada

  5. Central Research Lab., Taiyo Kagaku Co. Ltd., Yokkaichi, 510, Japan

    Yuko Enoki & Lekh Raj Juneja

Authors
  1. Shin Yazawa
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  2. Naohisa Kochibe
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  3. Toyo Nishimura
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  4. Chikako Shima
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  5. Izumi Takai
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  6. Masakazu Adachi
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  7. Takayuki Asao
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  8. Toshikazu Hada
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  9. Yuko Enoki
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  10. Lekh Raj Juneja
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Yazawa, S., Kochibe, N., Nishimura, T. et al. A novel method for determination of α1,6fucosyltransferase activity using a reducing oligosaccharide from egg yolk as a specific acceptor. Glycoconj J 15, 863–871 (1998). https://doi.org/10.1023/A:1006954915102

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