Molecular and Cellular Biochemistry

, Volume 198, Issue 1–2, pp 151–156 | Cite as

Vitamin E-deficiency induced changes in ovary and uterus

  • Padma Das
  • Mridula Chowdhury


Female rats at weaning (30 days age) were maintained on vitamin E-deficient diet for 70 days. The vitamin E-deficient and control animals were sacrified on 100 days of age. To study recovery a group of animals were supplemented with normal diet for last 25 days after initial 45 days of deficient diet or vice versa. The most striking data found were (i) significant drop in uterine weight in deficient group (ii) significant decrease in estrogen, LH and estrogen-induced uterine enzymes alkaline phosphatase and peroxidase and (iii) ovarian dysfunction as noted by degenerating graffian follicles. The significance of these findings is discussed in this report.

vitamin E-deficiency ovary urerus alkaline phosphatase peroxidase 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Evans HM, Bishop KS: On the existence of a hitherto unrecognized dictatory factor essential for reproduction. Science 56: 650–651, 1922Google Scholar
  2. 2.
    Pan SY, Van Dyke HB, Kaunitz H, Slanetz CA: Effect of vitamin Edeficiency on amount of gonadotrophin in the anterior pituitary of rats. Proc Soc Exp Biol Med 72: 325–326, 1949Google Scholar
  3. 3.
    Beerstecher E: Estrogens in the urine of normal and vitamin E-depleted rats. Endocrinol 28: 344, 1941Google Scholar
  4. 4.
    Freed M: Vitamin-E (Tocopherols). Methods of vitamin assay. Third edn. 363–402, 1966Google Scholar
  5. 5.
    Keyden HS, Chow CK, Bjornson LK: Spectrophotometric method for determination of tocopherol in red blood cells. J Lipid Res 14: 533–540, 1973PubMedGoogle Scholar
  6. 6.
    Emmerie A, Engel C: Des Pays-Bas et de la Belgique. Rec Tray Chim 57: 1351–1352, 1938Google Scholar
  7. 7.
    Chowdhury M, Sarkar M: Estradiol Benzoate induced changes in protein synthesis and lysosomal hydrolases in the pituitary of male rats. Biochem Biophys Res Comm 116: 236, 1983Google Scholar
  8. 8.
    Bessey A, Lowry OH, Brock MJ: A method for the rapid determination of Alkaline phosphatase with five cubic millimeters of serum. J Biol Chem 164: 321–329, 1946Google Scholar
  9. 9.
    Avrameads S: Immunoenzymeic techniques for biomedical analysis methods in enzymology, XLIV. Academic Press, New York, 1976, pp 709–716Google Scholar
  10. 10.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275, 1951PubMedGoogle Scholar
  11. 11.
    Chowdhury M, Tcholakian RK, Steinberger E: Effects of oestradiol benzoate (OeB) and human chorionic gonadotropin (hcG) on the testis and accessary sex glands of the rat. Acta Endocrinol 96: 273–280, 1981PubMedGoogle Scholar
  12. 12.
    Chowdhury M, Kabir SN, Pal AK, Pakrashi A: Modulation of luteinizing hormone receptors, an effect of an inhibitor of prolactin secretion, P-Caumaric acid. J Endocrinol 98: 307–311, 1983PubMedGoogle Scholar
  13. 13.
    Steel RGD, Torrie JH: Principles and procedures of statistics. McGraw-Hill, New York, 1960Google Scholar
  14. 14.
    Martin AJP, Moore T: Some effects of prolonged vitamin-E deficiency in the rat. J Hygiene 39: 643–645, 1939Google Scholar
  15. 15.
    Pan SY: Restoration of vaginal estrus by Alpha-tecopherol acetate in old rats. Ann NY Acad Sci 52: 80, 1949Google Scholar
  16. 16.
    Lyttle C, Desombre R: Uterine Peroxidase as a marker for estrogen action. Proc Natl Acad Sci USA 74(8): 3162–3166, 1977PubMedGoogle Scholar
  17. 17.
    Manning JP, Steinetz BG, Giannina T: Decidual alkaline phosphatase activity in the pregnant and pseudopregnant rat. Ann NY Acad Sci 166: 482–509, 1969PubMedGoogle Scholar
  18. 18.
    Cooper DR, Kling OR, Carpenter MP: Effect of vitamin E deficiency on serum concentrations of follicle stimulating hormone and testosterone during testicular maturation and degeneration. Endocrinology 120: 83–90, 1987PubMedGoogle Scholar
  19. 19.
    Akazawa N, Mikarni S, Kimura S: Effects of vitamin E deficiency on the hormone secretion of the pituitory-gonadal axis of the rat. Tohoku J Exp Med 152(3): 221–229, 1987PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Padma Das
    • 1
  • Mridula Chowdhury
    • 1
  1. 1.Indian Institute of Chemical BiologyJadavpur, CalcuttaIndia

Personalised recommendations