Abstract
To elucidate the effect of a modification of ceramide on antigenicity of the carbohydrate of ganglioside, the reactivity of O-acetyl GM3 having 3-O-acetyl ceramide, which has been characterized as a gliomarelated ganglioside, with monoclonal antibody M2590 was examined in comparison to that of non-acetylated GM3, by means of quantitative enzyme-linked immunosorbent assay, TLC-immunostaining and liposome immune lysis assay. In all these assay systems, O-acetyl GM3 showed less activity than GM3 as follows: GM3 was detected till 0.1 nmol in TLC-immunostaining, whereas O-acetyl GM3 could not be detected even at 0.25 nmol; the GM3 reaction was approximately twofold that of O-acetyl GM3 at each diluted point in the enzyme-linked immunosorbent assay; and 20% of the liposomes containing GM3 were lysed at 6 mol%, while liposomes containing O-acetyl GM3 did not lyse at that concentration. The lesser antigenicity of the sugar moiety of O-acetyl GM3 could be ascribed to the presence of an acetyl group in the ceramide at the 3-position of sphingosine.
Similar content being viewed by others
References
Hakomori S (1981) Ann Rev Biochem 50: 733-64.
Kannagi R, Nudelman E, Hakomori S (1982) Proc Natl Acad Sci USA 79: 3470-74.
Itonori S, Hidari K, Sanai Y, Taniguchi M, Nagai Y (1989) Glycoconj J 6: 551-60.
Nudelman E, Hakomori S, Kannagi R, Levery S, Yeh MY, Hellstrom KE, Hellstrom I (1982) J Biol Chem 257: 12752-56.
Nakakuma H, Arai M, Kawaguchi T, Horikawa K, Hidaka M, Sakamoto K, Iwamori M, Nagai Y, Takatsuki K (1989) FEBS Lett 258: 230-32.
Obeid LM, Linardic CM, Karolak LA, Hannun YA (1993) Science 259: 1769-71.
Ji L, Zhang G, Uematsu S, Akahori Y, Hirabayashi Y (1995) FEBS Lett 358: 211-14.
Suetake K, Tsuchihashi K, Inaba K, Chiba M, Ibayashi Y, Hashi K, Gasa S (1995) FEBS Lett 361: 201-05.
Tsuchihashi K, Daino T, Akino T, Gasa S (1996) J Lipid Res 37: 2136-44.
Hirabayashi Y, Hamaoka A, Matsumoto M, Matsubara T, Tagawa M, Wakabayashi S, Taniguchi M (1985) J Biol Chem 260: 13328-33.
Gasa S, Makita A, Kinoshita Y (1983) J Biol Chem 258: 876-81.
Gasa S, Makita A, Yanagisawa K, Nakamura M (1984) Adv Exp Med Biol 174: 111-17.
Gasa S, Kamio K, Makita A (1992) J Lipid Res 33: 1079-84.
Yu RK, Ledeen RW (1970) J Lipid Res 11: 506-16.
Higashi H, Fukui Y, Ueda S, Kato S, Hirabayashi Y, Matsumoto M, Naiki M (1984) J Biochem Tokyo 95: 1517-20.
Tai T, Cahan LD, Paulson JC, Saxton RE, Irie RF (1984) J Natl Cancer Inst 73: 627-33.
Nores GA, Dohi T, Taniguchi M, Hakomori S (1987) J Immunol 139: 3171-76.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Daino, T., Tsuchihashi, K., Kashiwagi, M. et al. Antigenicity of the carbohydrate moiety of ganglioside GM3 having 3-O-acetyl ceramide. Glycoconj J 16, 39–43 (1999). https://doi.org/10.1023/A:1006949719565
Issue Date:
DOI: https://doi.org/10.1023/A:1006949719565