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Structures of the N-linked carbohydrate of ascorbic acid oxidase from zucchini

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Abstract

N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J 5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoproteins. To prevent misinterpretations of immunological data, the structure of the N-glycan of ascorbic acid oxidase has been reinvestigated. The oligosaccharides were released by almond N-glycosidase and analysed as their pyridylamino derivatives by 2D-HPLC and exoglycosidase digestions. The main structure resembled the typical complex plant N-glycan consisting of a core-pentasaccharide decorated with xylose and 3-linked fucose. The other abundant species lacked the fucose residue. Small amounts of these glycans carried a GlcNAc residue on the 6-arm. Therefore, ascorbic acid oxidase will not only react with antibodies directed against the xylosylated region but also with those binding to N-glycans with 3-linked fucose.

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  1. Institut fur Chemie, Universitat fur Bodenkultur, Muthgasse 18, A-1190, Wien, Austria

    Friedrich Altmann

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  1. Friedrich Altmann
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Altmann, F. Structures of the N-linked carbohydrate of ascorbic acid oxidase from zucchini. Glycoconj J 15, 79–82 (1998). https://doi.org/10.1023/A:1006947701714

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