Skip to main content
SpringerLink
Log in

Mitochondrial and mitochondia-related nuclear genetic function in rabbit urinary bladder following reversal of outlet obstruction

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Partial outlet obstruction of the rabbit urinary bladder causes increased tissue hypertrophy and decreased contractility of that organ; we showed that, in an experimental rabbit model, both correlate closely with alterations in the status and expression of mitochondrial (mt), and mt-related nuclear, genetic parameters in bladder smooth muscle. Here we investigate the rate and overall level of recovery of mt and nuclear genetic function following reversal of outlet obstruction in the same animal model. Release from outlet obstruction at 28 days resulted in improvement in both level of hypertrophy and contractile function in all bladders studied. However, bladders fell into two groups based on whether relative copy mt genome number per cell was above or below that of unobstructed controls. Bladders with high mt DNA content adjusted organellar genome copy number toward normal post-reversal but did not properly adjust mt transcript levels; mt-related nuclear transcripts in these samples showed recovery. Bladders with low mt DNA content showed no adjustment of those levels toward normal post-reversal but did show some adjustment in other mt and nuclear genetic parameters. Thus, a limiting factor for return of normal bladder function following reversal of outlet obstruction may be recovery of normal mt genetic performance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sterling AM, Ritter RC, Zinner NR: The physical basis of obstructive uropathology. In: F Hinman (ed). Benign Prostafic Hypertrophy. Springer-Verlag, New York, 1983. pp 433–442

    Google Scholar 

  2. Levin RM, Longhurst PA, Monson FC, Haugaard N, Wein AJ: Experimental studies on bladder outlet obstruction. In: H Lepor, RK Lawson (eds). Prostate Diseases. WB Saunders, Philadelphia, 1993. pp 119–130

    Google Scholar 

  3. Ghoniem GM, Regnier CH, Biancani P, Johnson L, Susset JG: Effect of vesical outlet obstruction on detrusor contractility and passive properties in rabbits. J Urol 135: 1284–1289, 1986

    Google Scholar 

  4. Levin RM, Longhurst PA, Monson FC, Kato K, Wein AJ: Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder. Prostate (Supp 3): 9–26, 1990

  5. Bilgen A, Wein AJ, Haugaard B, Packard D, Levin RM: Effect of outlet obstruction on pyruvate metabolism of the rabbit urinary bladder. Mol Cell Biochem 117: 159–163, 1992

    Google Scholar 

  6. Haugaard N, Potter L, Wein AJ, Levin RM: Effect of partial outlet obstruction of the rabbit urinary bladder on malate dehydrogenase and citrate synthase activity. J Urol 147: 1391–1393, 1992

    Google Scholar 

  7. Levin RM, Haugaard N, Levin SS, Wein AJ: Creatine kinase activity in normal and hypertrophied rabbit urinary bladder tissue (following partial outlet obstruction). Mol Cell Biochem 106: 143–149, 1991

    Google Scholar 

  8. Buttyan R, Jacobs B, Blaivis JG, Levin RM: The early molecular response to rabbit bladder outlet obstruction. Neurourol Urodynam 11: 253–260, 1992

    Google Scholar 

  9. Zhao Y, Chacko S, Levin RM: Expression of stress proteins (HSP-70 and HSP-90) in the rabbit urinary bladder subjected to partial outlet obstruction. Mol Cell Biochem 130: 49–55, 1994

    Google Scholar 

  10. Zhao Y, Levin RM, Levin SS, Nevel CA, Haugaard N, Hueih-Shing Hsu T, Hudson AP: Partial outlet obstruction of the rabbit bladder results in changes in the mitochondrial genetic system. Mol Cell Biochem 141: 47–55, 1994

    Google Scholar 

  11. Nevel-McGarvey CA, Levin RM, Hudson AP: Transcription of mitochondrial and mitochondria-related nuclear genes in rabbit bladder following partial outlet obstruction. Mol Cell Biochem 173: 95–102, 1997

    Google Scholar 

  12. Nagley P: Coordination of gene expression in the formation of mammalian mitochondria. Trends Genet 7: 1–4, 1991

    Google Scholar 

  13. Levin RM, Malkowicz SB, Wein AJ, Atta MA, Elbadawi A: Recovery from short term obstruction of the rabbit urinary bladder. J Urol 134: 388–390, 1985

    Google Scholar 

  14. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989

    Google Scholar 

  15. Horikoshi T, Danenberg K, Volkenandt M, Stadibauer T, Danenberg PV: Quantitative measurement of relative gene expression in human tumors. In: B.A. White (ed). Methods in Molecular Biology, Vol 15; PCR Protocols: Current Methods and Applications. Humana Press lnc, Totowa, NJ, 1993. pp 177–188

    Google Scholar 

  16. McDaniel T, Meltzer SJ: Direct radioactive labeling of polymerase chain reaction products. In: BA White (ed). Methods in Molecular Biology, Vol 15; PCR Protocols: Current Methods and Applications. Humana Press, Totowa, NJ, 1993. p 41–43

    Google Scholar 

  17. Gross-Bellard M, Oudet P, Chambon P: Isolation of high molecular weight DNA from mammalian cells. Eur J Biochem 36: 32–38, 1972

    Google Scholar 

  18. Robinson JB, Breut LG, Sumegi B, Srere PA: An enzymatic approach to the study of the Krebs tricarboxylic acid cycle. In: VM Darley-Usmar, D Rickwood, MT Wilson (eds). Mitochondria: A Practical Approach. IRL Press, Oxford, 1989. pp 137–145

    Google Scholar 

  19. Daley-Usmar VM, Capaldi RA, Takamiya S, Millet F, Wilson MT, Malatesta F, Sarti P: Reconstitution and molecular analysis of the respiratory chain. In: V.M. Darley-Usmar, D. Rickwood, M.T. Wilson (eds). Mitochondria: A Practical Approach. IRL Press, Oxford, 1989. pp. 137–145

    Google Scholar 

  20. Lowry OM, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 268–271, 1951

    Google Scholar 

  21. Jeon KW, Friediander M: Mitochondrial Genomes. lnt Rev Cytol, Vol 141. Academic Press, NY, 1992

    Google Scholar 

  22. Monson FC, Wein AJ, Eika B, Murphy M, Levin RM: Stimulation of DNA synthesis in rabbit bladder wall after partial obstruction and acute overdistension. Neurourol Urodynam 13: 51–62, 1994

    Google Scholar 

  23. Clayton D: Replication and transcription of the mammalian mitochondrial genome. Annu Rev Biochem 53: 575–594, 1991

    Google Scholar 

  24. Ropp PA, Copeland WC: Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase γ. Genomics 36: 449–458, 1996

    Google Scholar 

  25. Shadel GS, Clayton DA: Mitochondrial DNA maintenance in vertebrates. Annu Rev Biochem 66: 409–435, 1997

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, MCP-Hahnemann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, PA, USA

    Christina A. Nevel-McGarvey

  2. Division of Urology, Department of Surgery, University of Pennsylvania, School of Medicine, Philadelphia, PA

    Dorothea Rohrmann

  3. Albany College of Pharmacy, Albany, NY, USA

    Robert M. Levin

  4. Medical Research Service, Veterans Affairs Medical Centre, University and Woodland Avenues, Philadelphia, PA, USA

    Alan P. Hudson

Authors
  1. Christina A. Nevel-McGarvey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dorothea Rohrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert M. Levin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alan P. Hudson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nevel-McGarvey, C.A., Rohrmann, D., Levin, R.M. et al. Mitochondrial and mitochondia-related nuclear genetic function in rabbit urinary bladder following reversal of outlet obstruction. Mol Cell Biochem 197, 161–172 (1999). https://doi.org/10.1023/A:1006945732718

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006945732718

Search

Navigation