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Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) gene: Structure, organization and regulation by phorbol ester, a protein kinase C activator

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Abstract

At present there are two recognized members of the ROS-GC subfamily of membrane guanylate cyclases. They are ROS-GC1 and ROS-GC2. A distinctive feature of this family is that its members are not switched on by the extracellular peptide hormones; instead, they are modulated by intracellular Ca2+ signals, consistent to their linkage with phototransduction. An intriguing feature of ROS-GC1, which distinguishes it from ROS-GC2, is that it has two Ca2+ switches. One switch inhibits the enzyme at micromolar concentrations of Ca2+, as in phototransduction; the other, stimulates. The stimulatory switch, most likely, is linked to retinal synaptic activity. Thus, ROS-GC1 is linked to both phototransduction and the synaptic activity. The present study describes (1) the almost complete structural identity of 18.5 kb ROS-GC1 gene; (2) its structural organization: the gene is composed of 20 exons and 19 introns with classical GT/AG boundaries; (3) the activity of the ROS-GC1 promoter assayed through luciferase reporter in COS cells; and (4) induction of the gene by phorbol ester, a protein kinase C (PKC) activator. The co-presence of PKC and ROS-GC1 in photoreceptors suggests that regulation of the ROS-GC1 gene by PKC might be a physiologically relevant phenomenon.

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Authors and Affiliations

  1. The Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology, University of Medicine and Dentistry of New Jersey, Stratford, NJ, USA

    Teresa Duda, Venkateswar Venkataraman, Anuradha Krishnan & Rameshwar K. Sharma

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  1. Teresa Duda
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  2. Venkateswar Venkataraman
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  3. Anuradha Krishnan
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  4. Rameshwar K. Sharma
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Duda, T., Venkataraman, V., Krishnan, A. et al. Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) gene: Structure, organization and regulation by phorbol ester, a protein kinase C activator. Mol Cell Biochem 189, 63–70 (1998). https://doi.org/10.1023/A:1006944629935

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