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Studies on hepatic oxidative stress and antioxidant defence systems during arteether treatment of Plasmodium yoelii nigeriensis infected mice

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Abstract

Reactive Oxygen species play an important role in pathology during malaria infection. The status of hepatic oxidative stress and antioxidant defence indices was studied during Plasmodium yoelii nigeriensis (P. y. nigeriensis) infection in mice and arteether treatment of P. y. nigeriensis infected mice. P. y. nigeriensis infection caused a significant increase in hepatic xanthine oxidase, rate of lipid peroxidation, reduced glutathione (GSH) and glutathione reductase with progressive rise in parasitemia. This was accompanied by a significant decrease in hepatic superoxide dismutase (SOD) and catalase with increase in parasitemia. Arteether treatment (10 mg/kg body weight of mice) of infected mice from day 2 of post infection resulted in complete clearance of parasitemia on day 4 of post infection which was accompanied by restoration of all the oxidative stress and antioxidant defence indices to normal levels.

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References

  1. World Health Organization, Malaria Unit: Global malaria control. Bull WHO 71: 281–284, 1993

    Google Scholar 

  2. Meshnick SR, Taylor TE, Kamchonwongpaisan S: Artemisinin and the antimalarial endoperoxides: From herbal remedy to targeted chemotherapy. Microbiol Rev 60: 301–315, 1996

    Google Scholar 

  3. Clark IA, Chaudhri G, Cowden WB: Some roles of free radicals in malaria. Free Rad Biol Med 6: 315–321, 1989

    Google Scholar 

  4. Srivastava P, Puri SK, Dutta GP, Pandey VC: Hepatic superoxide scavenging system during Plasmodium berghei infection and chloroquine treatment. Med Sci Res 19: 307–308, 1991

    Google Scholar 

  5. Meister A, Anderson ME: Glutathione. Ann Rev Biochem 52: 711–760, 1983

    Google Scholar 

  6. Lai H, Singh NP: Selective cancer cell cytotoxicity from exposure to dihydroartemisinin and halotransferrin. Cancer Lett 91: 41–46, 1995

    Google Scholar 

  7. Utley HG, Bernheim F, Hochstein P: Effect of sulfhydryl reagent on peroxidation in microsomes. Arch Biochem Biophys 118: 29–32, 1967

    Google Scholar 

  8. Aebi H: Catalase in vitro: In: Meth Enzymol 105. Academic Press, London, 1984, pp 121–126

    Google Scholar 

  9. Beutler E, Duran O, Kelly BM: Improved method of determination of blood glutathione. J Lab Clin Med 61: 882–888, 1963

    Google Scholar 

  10. Racker E: Glutathione reductase. In: Meth in Enzymol II. Academic Press, New York, 1955, pp 722–725

    Google Scholar 

  11. Kakkar P, Das B, Vishwanathan PN: A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophys 21: 130–132, 1984

    Google Scholar 

  12. Nishikimi M, Appaji Raeo N, Yaki K: The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 46: 849–854, 1972

    Google Scholar 

  13. Chander R, Lapoor NK, Dhawan BN: Picrolive, picroside I and kutkoside from Picrorhiza kurroa are scavengers of superoxide anions. Biochem Pharmacol 44: 180–183, 1992

    Google Scholar 

  14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275, 1951

    Google Scholar 

  15. Jervis HR, MacCallum DK, Sprinz H: Experimental P. berghei infection in hamster: Its effects on the liver. Arch Pathol 86: 328–337, 1968

    Google Scholar 

  16. Aikawa JE, Suzu M, Gutierez Y: Pathology of malaria. In: JP Krier (ed). Malaria Volume 2. Academic Press, New York, 1980, pp 47–102

    Google Scholar 

  17. Maegraith BG, Skirrow MB, Changsuphajassidhi T: Liver blood flow in Plasmodium knowlesi malaria. Trans R Soc Trop Med Hyg 57: 1, 1963

    Google Scholar 

  18. Pandey VC, Dutta GP, Saxena N, Srivastava R, Ghatak SN: Macaca mullata - a model for studying host parasite relationship in malaria. In: KR Bhardwaj, BN Dhawan (eds). Non-human Primates in Biomedical Research. CDRI, Lucknow, 1986, pp 111–126

    Google Scholar 

  19. White NJ: The pathology of Malaria. In: Advances in Parasitology 31. Academic Press, London, 1992, pp 130–131

  20. Ezio T, Bruno Lotti, Cavallo G, Croce C, Borelli G: Liver xanthine oxidase increase in three pathological models. A possible defence mechanism. Biochem Pharmacol 29: 1939–1943, 1980

    Google Scholar 

  21. Gutteridge JMC, Halliwell B: The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem 5: 129–135, 1990

    Google Scholar 

  22. Guillemette J, Marion M, Denizeau F, Fournier M, Brosseau P: Characterization of the in vitro hepatocyte model for toxicological evaluation: Repeated growth stimulation and glutathione response. Biochem Cell Biol 71: 7–13, 1993

    Google Scholar 

  23. Kano Y, Fridovich I: Superoxide radical inhibits catalase. J Biol Chem 257: 5751–5754, 1982

    Google Scholar 

  24. Gu HM, Warhurst DC, Peters W: Uptake of [3H] dihydroartemisinin by erythrocytes infected with Plasmodium falciparum in vitro. Trans R Soc Trop Med Hyg 78: 265–270, 1984

    Google Scholar 

  25. Meshnick SR: Free radicals and antioxidants. Lancet 344: 1441–1442, 1994

    Google Scholar 

Download references

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  1. Division of Biochemistry, Central Drug Research Institute, Lucknow, 226 001, India E-mail

    Nikhat J. Siddiqi & V.C. Pandey

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  1. Nikhat J. Siddiqi
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  2. V.C. Pandey
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Siddiqi, N.J., Pandey, V. Studies on hepatic oxidative stress and antioxidant defence systems during arteether treatment of Plasmodium yoelii nigeriensis infected mice. Mol Cell Biochem 196, 169–173 (1999). https://doi.org/10.1023/A:1006944313291

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  • DOI: https://doi.org/10.1023/A:1006944313291

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