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Lignin peroxidase isoforms from Streptomyces viridosporus T7A: are they a monomer based structure?

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Biotechnology Techniques

Abstract

Five fractions with lignin peroxidase activity were isolated by FPLC-Mono Q from a Streptomyces viridosporus culture. F4 and F5 showed the highest specific activity and degree of protein homogeneity by chromatofocusing, IEF- and gradient-PAGE. The individual analysis of F4 and F5 by FPLC-Superdex 75, showed MW that were multiples to each other (68,000; 23,000; 12,000), although by SDS PAGE a sole MW of 13,500 was obtained, indicating a monomer based structure. The amino-acid composition of F5 showed absence of sulfur amino acids.

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Authors and Affiliations

  1. Instituto de Química, Depto. de Bioquímica, Universidade Federal do Rio de Janeiro, CT, Bloco A, Ilha do Fundão, Rio de Janeiro, RJ, CEP 21949-900, Brasil

    Elba P.S. Bon, Hilton J. Nascimento & José G. Silva Jr.

  2. Depto. de Bioquímica, IBRAG, Universidade do Estado do Rio de Janeiro, RJ, CEP 20551-013, Brasil

    Jacyara M.B. Macedo

Authors
  1. Elba P.S. Bon
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  2. Hilton J. Nascimento
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  3. Jacyara M.B. Macedo
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  4. José G. Silva Jr.
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Bon, E.P., Nascimento, H.J., Macedo, J.M. et al. Lignin peroxidase isoforms from Streptomyces viridosporus T7A: are they a monomer based structure?. Biotechnology Techniques 13, 289–293 (1999). https://doi.org/10.1023/A:1006914312208

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  • DOI: https://doi.org/10.1023/A:1006914312208

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