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A possible role of Schisto FLRFamide in inhibition of adipokinetic hormone release from locust corpora cardiaca

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Journal of Neurocytology

Abstract

The distribution and actions of FMRFamide-related peptides (FaRPs) in the corpora cardiaca of the locust Locusta migratoria were studied. Antisera to FMRFamide and SchistoFLRFamide (PDVDHVFLRFamide) label neuronal processes that impinge on glandular cells in the glandular lobe of the corpora cardiaca known to produce adipokinetic hormones. Electron microscopic immunocytochemistry revealed that these FaRP-containing processes form synaptoid contacts with the glandular cells. Approximately 12% of the axon profiles present in the glandular part of the corpus cardiacum contained SchistoFLRFamide-immunoreactive material. Retrograde tracing of the axons in the nervus corporis cardiaci II with Lucifer yellow revealed 25–30 labelled neuronal cell bodies in each lateral part of the protocerebrum. About five of these in each hemisphere reacted with the SchistoFLRFamide-antiserum. Double-labelling immunocytochemistry showed that the FaRP-containing processes in the glandular lobe of the corpora cardiaca are distinct from neuronal processes, reacting with an antiserum to the neuropeptide locustatachykinin. The effect of the decapeptide SchistoFLRFamide and the tetrapeptide FMRFamide on the release of adipokinetic hormone I (AKH I) from the cells in the glandular part of the corpus cardiacum was studied in vitro. Neither the deca- nor the tetrapeptide had any effect on the spontaneous release of AKH I. Release of AKH I induced by the phosphodiesterase inhibitor IBMX, however, was reduced significantly by both peptides. These results point to an involvement of FaRPs as inhibitory modulators in the regulation of the release of adipokinetic hormone from the glandular cells.

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Authors and Affiliations

  1. Department of Experimental Zoology, Utrecht University, Padualaan 8, NL-3584 CH, Utrecht, The Netherlands

    H. G. B. Vullings, S. E. C. G. Ten Voorde, P. C. C. M. Passier, J. H. B. Diederen & D. J. Van Der Horst

  2. Department of Zoology, Stockholm University, Svante Arrhenius väg 16, S-10691, Stockholm, Sweden

    D. R. NÄSSEL

Authors
  1. H. G. B. Vullings
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  2. S. E. C. G. Ten Voorde
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  3. P. C. C. M. Passier
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  4. J. H. B. Diederen
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  5. D. J. Van Der Horst
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  6. D. R. NÄSSEL
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Correspondence to D. R. NÄSSEL.

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Vullings, H.G.B., Ten Voorde, S.E.C.G., Passier, P.C.C.M. et al. A possible role of Schisto FLRFamide in inhibition of adipokinetic hormone release from locust corpora cardiaca. J Neurocytol 27, 901–913 (1998). https://doi.org/10.1023/A:1006901123566

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