Skip to main content
SpringerLink
Log in

Histidine and histamine metabolism in rat enterocytes

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

To study the metabolic fate of L-histidine and histamine in rat isolated enterocytes, enterocytes were incubated in the presence of 0.1 mM L-[U-14C] histidine. At the rate of 11.1 ± 2.7 pmol/106 cells/30 min, the amino acid was incorporated into cellular proteins. 80 µM cycloheximide, i.e. a protein synthesis inhibitor, inhibited this incorporation by 70 ± 17%. L-histidine was used for cellular protein synthesis which depended on time and concentration. 0.1 mM L-[U-14C] histidine was little oxidized by intestinal cells, i.e. 0.12 ± 0.06 pmol/106 cells/30 min, and was not converted into histamine. When 10 mM histamine was added to the incubation medium, it completely inhibited the incorporation of 0.1 mM [1,4-14C] putrescine into isolated enterocytes. In enterocyte homogenates, this corresponded to inhibition by histamine of putrescine incorporation as catalyzed by transglutaminase activity. Since histamine incorporation into TCA-precipitable material derived from enterocyte homogenates depended on time and concentration, we concluded that exogenous, but not de novo-formed histamine was able to compete with putrescine incorporation into enterocytes as catalyzed by transglutaminase activity. (Mol Cell Biochem 175: 143–148, 1997)

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Windmueller HG, Spaeth AE: Respiratory fuels and nitrogen metabolism in vivo in small intestine of fed rats. J Biol Chem 255: 107–112, 1980

    Google Scholar 

  2. Blachier F, Darcy-Vrillon B, Sener A, Duée PH, Malaisse WJ: Arginine metabolism in rat enterocytes. Biochim Biophys Acta 1092: 304-310, 1991

    Google Scholar 

  3. Argiles JM, Lopez-Soriano FJ: Intestinal amino acid transport: an overview. Int J Biochem 22: 931–937, 1990

    Google Scholar 

  4. Huneau JF, Tomé D, Wal JM: Histamine content, diamine oxidase and histidine decarboxylase activities along the intestinal tract of the rat. Agents and Actions 28: 231–234, 1989

    Google Scholar 

  5. Rangachari PK: Histamine: mercurial messenger in the gut. Am J Physiol 262: G1–G13, 1992

    Google Scholar 

  6. Sener A, Dunlop ME, Gomis R, Mathias PCF, Malaisse-Lagae F, Malaisse WJ: Role of transglutaminase in insulin release. Study with glycine and sarcosine methylesters. Endocrinology 117: 237–242, 1985

    Google Scholar 

  7. Sener A, Blachier F, Rasschaert J, Malaisse WJ: Stimulus-secretion coupling of arginine-induced insulin release: comparison with histidine-induced insulin release. Endocrinology 127: 107–113, 1990

    Google Scholar 

  8. Blachier F, Mourtada A, Gomis R, Sener A, Malaisse WJ: Metabolic and secretory response of parotid cells to cationic amino acids. Uptake and catabolism of L-arginine and L-ornithine. Biochim Biophys Acta 1091: 151–157, 1991

    Google Scholar 

  9. Folk JE: Transglutaminases. Ann Rev Biochem 49: 517–531, 1980

    Google Scholar 

  10. Greenberg CS, Birckbichler PJ, Rice RH: Transglutaminases: multifunctional crosslinking enzymes that stabilize tissues. FASEB J 5: 3071–3077, 1991

    Google Scholar 

  11. Kumagai J, Johnson LR: Characteristics of putrescine uptake in isolated rat enterocytes. Am J Physiol 254: G81–G86, 1988

    Google Scholar 

  12. Kumagai J, Jain R, Johnson LR: Characteristics of spermidine uptake by isolated rat enterocytes. Am J Physiol 256: G905–G910, 1989

    Google Scholar 

  13. Kummerlen C, Seiler N, Galluser M, Gossé F, Knödgen B, Hasselman M, Raul F: Polyamines and the recovery of intestinal morphology and function after ischemic damage in rats. Digestion 55: 168–174, 1994

    Google Scholar 

  14. Kvietys PR, Specian RD, Cepinskas G: Polyamines attenuate jejunal mucosa injury induced by oleic acid. Am J Physiol 263: G224–G229, 1992

    Google Scholar 

  15. Luk GD, Marton LJ, Baylin SB: Ornithine decarboxylase is important in intestinal mucosa maturation and recovery from injury in rats. Science 210: 195–198, 1980

    Google Scholar 

  16. Fujimoto K, Granger DN, Price VH, Tso P: Ornithine decarboxylase is involved in repair of small intestine after ischemia-reperfusion in rats. Am J Physiol 261: G523–G529, 1991

    Google Scholar 

  17. D'Argenio G, Sorrentini I, Cosenza V, Gatto A, Iovino P, D'Armiento FP, Baldassare F, Mazzacca G: Serum and tissue transglutaminase correlates with the severity of inflammation in induced colitis in the rat. Scand J Gastroenterol 27: 111–114, 1992

    Google Scholar 

  18. Wang JY, Viar MJ, Johnson LR: Transglutaminase in response to hypertonic NaCl induced gastric mucosal injury in rats. Gastroenterology 104: 65–74, 1993

    Google Scholar 

  19. Seiler N, Knödgen B: High performance liquid chromatographic procedure for the simultaneous determination of the natural polyamines and their monoacetyl derivatives. J Chromatogr 221: 227–235, 1980

    Google Scholar 

  20. Blachier F, M'Rabet-Touil H, Posho L, Morel MT, Bernard F, Darcy-Vrillon B, Duée PH: Polyamine metabolism in enterocytes isolated from newborn pigs. Biochim Biophys Acta 1175: 21–26, 1992

    Google Scholar 

  21. Adibi SA, Mercer DW: Protein digestion in human intestine as reflected in luminal, mucosal, and plasma amino acid concentrations after meals. J Clin Invest 52: 1586–1594, 1973

    Google Scholar 

  22. Fujisaki J, Fujimoto K, Oohara A, Sakata T, Hirano M, Takaski O, Iwakiri R, Yamaguchi M: Roles of histamine and diamine oxidase in mucosa of rat small intestine after ischmia-reperfusion. Dig Dis Sci 38: 1195–1200, 1993

    Google Scholar 

  23. Hiramatsu Y, Okabe S: Effect of intraduodenally administered histamine on gastric acid secretion in rats and guinea pigs. Dig Dis Sci 39: 689–697, 1994

    Google Scholar 

  24. Hogan DL, Yao B, Barrette KE, Isenberg JI: Histamine inhibits prostaglandin E2-stimulated rabbit duodenal bicarbonate secretion via H2 receptors and enteric nerves. Gastroenterology 108: 1676–1682, 1995

    Google Scholar 

  25. Keely SJ, Stack WA, O'Donoghue DP, Baird AW: Regulation of ion transport by histamine in human colon. Eur J Pharmacol 279: 203–209, 1995

    Google Scholar 

  26. Bruce SE, Bjarnason I, Peters TJ: Human jejunal transglutaminase: demonstration of activity, enzyme kinetics and substrate specificity with special relation to gliadin and coeliac disease. Clin Sci 68: 573–579, 1985

    Google Scholar 

  27. Patel EK, Bruce SE, Bjarnasson I, Peters TJ: Rat gastrointestinal transglutaminase: demonstration of enzyme activity and cell and tissue distribution. Cell Biochem Funct 3: 199–203, 1985

    Google Scholar 

  28. D'Argenio G, Sorrentini I, Ciacci C, Mazzacca G: Transglutaminase activity along the rat small bowel and cellular location. Enzyme 39: 227–230, 1988

    Google Scholar 

  29. M'Rabet-Touil H, Blachier F, Hellio N, Robert V, Cherbuy C, Darcy-Vrillon B, Duée PH: Transglutaminase activity in enterocytes isolated from pig jejunum. Mol Cell Biochem 146: 49–54, 1995

    Google Scholar 

  30. Bersten AM, Ahkong QF, Hallinan T, Nelson SJ, Lucy JA: Inhibition of the formation of myotubes in vitro by inhibitors of transglutaminase Biochim Biophys Acta 762: 429–436, 1983

    Google Scholar 

  31. Blachier F, Selamnia M, Robert V, M'Rabet-Touil H, Duée PH: Metabolism of L-arginine through polyamine and nitric oxide synthase pathways in proliferative or differentiated human colon carcinoma cells. Biochim Biophys Acta 1268: 255–262, 1995

    Google Scholar 

  32. Tsunada S, Fujimoto K, Gotoh Y, Sakai T, Kang M, Sakata T, Granger DN, Tso P: Role of histamine receptors in intestinal repair after ischemia-reperfusion in rats. Gastroenterology 107: 1297–1304, 1994

    Google Scholar 

  33. Fujimoto K, Sakata Y, Tsunada S, Koyama T, Morita H, Ogata SI, Matsunaga C, Gotoh Y, Iwakiri R: Newly synthesized histamine accelerates omithine decarboxylase activity in rat intestinal mucosa after ischemia-reperfusion. Dig Dis Sci 40: 717–721, 1995

    Google Scholar 

  34. Koyama T, Fujimoto K, Kang M, Yoshimatsu H, Sakata T, Tsunada S, Morita H, Iwakiri R, Sakai T: Histamine effect on ornithine decarboxylase of rat intestine in case of ischemia-reperfusion compared with refeeding. Proc Soc Exp Biol Med 209: 27–31, 1995

    Google Scholar 

  35. Wang JY, Johnson LR: Role of transglutaminase and protein crosslinking in the repair of mucosal stress erosions. Am J Physiol 262: G818–G825, 1992

    Google Scholar 

  36. Knutson L, Ahrenstedt O, Odlind B, Hällgren R: The jejunal secretion of histamine is increased in active Crohn's disease. Gastroenterology 98: 849–854, 1990

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Jouy-en-Josas, France

    Gwénaële Guihot & François Blachier

Authors
  1. Gwénaële Guihot
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. François Blachier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guihot, G., Blachier, F. Histidine and histamine metabolism in rat enterocytes. Mol Cell Biochem 175, 143–148 (1997). https://doi.org/10.1023/A:1006895931091

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006895931091

Search

Navigation