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Transcription of mitochondrial and mitochondria-related nuclear genes in rabbit bladder following partial outlet obstruction

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Abstract

Using the rabbit model, we showed that partial outlet obstruction of theurinary bladder causes significant changes in the status and expression ofthe mitochondrial (mt) genetic system in bladder smooth muscle immediatelyafter obstruction is initiated. Here we investigate quantitatively theseverity of the mt genetic response to partial outlet obstruction in bothshort- and long-term obstructed rabbits. Based on previous functionalstudies, bladders with mass < 6 fold greater than control were consideredcompensated; bladders with mass > 6 fold that of control were considereddecompensated. Analyses of DNA from compensated rabbit bladders showed thatrelative mt genome copy number decreased to 30% of control values.Transcript analyses for these samples showed that mt RNA levels increased 3fold to compensate for lower template copy number. Analysis of decompensatedbladders demonstrated that mt genome copy number increased to approximately90% of control levels; mt transcripts progressively decreased inthese samples by as much as 30 fold. In contrast, transcription of amt-related nuclear gene decreased 3-9 fold in compensated bladders butincreased 10-30 fold in decompensated bladders. Activity for the cytochromeoxidase complex, and for the mt enzyme citrate synthase, decreased steadilywith increasing bladder hypertrophy. These data suggest that bladderdysfunction following partial outlet obstruction is mediated partly by asignificant loss in mt and mt-related nuclear gene coordination.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, MCP-Hahnemann School of Medicine, Philadelphia, USA

    Christina A. Nevel-McGarvey & Alan P. Hudson

  2. Division of Urology, Department of Surgery, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA

    Robert M. Levin

Authors
  1. Christina A. Nevel-McGarvey
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  2. Robert M. Levin
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  3. Alan P. Hudson
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Nevel-McGarvey, C.A., Levin, R.M. & Hudson, A.P. Transcription of mitochondrial and mitochondria-related nuclear genes in rabbit bladder following partial outlet obstruction. Mol Cell Biochem 173, 1–8 (1997). https://doi.org/10.1023/A:1006890527552

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