Abstract
Cigarette smoking is an adverse prognostic factor for health. Its damaging effects on many enzymatic and cellular activities are well known. The present study was carried out to evaluate whether there is a difference in the binding of bilirubin to the erythrocytes from healthy smokers and non-smokers. The results suggest that the binding of bilirubin to the erythrocytes from healthy smokers as well as in vitro smoked erythrocytes is significantly higher than that of healthy non-smokers.
References
Meisel P, Jahr H: Influence of albumin-bound bilirubin on the antibodydependent cellular cytotoxicity of human cells in vitro. Biol Neonate 60: 308–313, 1991
Hayer M, Piva M-T, Sieso V, de Bornier BM: Experimental studies on unconjugated bilirubin binding by human erythrocytes. Clin Chim Acta 186: 345–350, 1989
Tayyab S, Ali MK: Binding of bilirubin to erythrocytes from different mammalian species. Comp Biochem Physiol 111A: 507–509, 1995
Sato H, Kashiwamata S: Interaction of bilirubin with human erythrocyte membranes. Biochem J 210: 489–496, 1983
Ohnishi S, Ito T: Calcium-induced phase separations in phosphatidylserine-phosphatidylcholine membranes. Biochemistry 13: 881–887, 1974
Karp WB, Subramanyam SB, Ho CK, Robertson AF: Drugs affecting bilirubin uptake by human erythrocyte ghosts. Am J Med Sci 289: 236–239, 1985
Niki E, Minamisawa S, Oikawa M, Komuro E: Membrane damage from lipid oxidation induced by free radicals and cigarette smoke. Ann NY Acad Sci 686: 29–38, 1993
Mukherjee S, Woods L, Weston Z, Das SK: The effect of mainstream and side stream cigarette smoke exposure on oxygen defence mechanisms of guinea pig erythrocytes. J Biochem Toxicol 8: 119–125, 1993
L'Abbe MR, Collins MW, Trick KD, Laffey PJ: Glutathione peroxidase activity in a healthy Canadian population. Effects of age, smoking and drinking habits, exercise and oral contraceptive use. Trace Elem Med 9: 45–53, 1992
Tayyab S, Qasim MA: Purification and properties of buffalo serum albumin. Biochem Int 20: 405–415, 1990
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-phenol reagent. J Biol Chem 193: 265–275, 1951
Fog J: Determination of bilirubin in serum as alkaline ‘azobilirubin’. Scand J Clin Lab Invest 10: 241–245, 1958
Oski FA, Naiman JL: Red cell binding of bilirubin. J Pediatr 63: 1034–1037, 1963
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Moin, S., Kutub Ali, M., Ullah Siddiqui, M. et al. Erythrocytes from healthy smokers bind more bilirubin than the erythrocytes from healthy non-smokers. Mol Cell Biochem 183, 211–214 (1998). https://doi.org/10.1023/A:1006888423008
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DOI: https://doi.org/10.1023/A:1006888423008