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27Al-NMR studies of aluminum transport across yeast cell membranes

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Abstract

Aluminum (Al) transport across yeast cells was studied using Dy(NO3)3 as a shift reagent by 27Al-NMR spectroscopy. The results showed that (a) Al enters the yeast cells at 15 min and over a period of time, within 4 h, an equilibrium sets in between outside and inside Al; (b) citrate does not favor Al going into the yeast cells at pH 5.0; and (c) EDTA brings out all the Al that has entered the yeast cells. (Mol Cell Biochem 175: 59–63, 1997)

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References

  1. Alfrey AC: The physiology of aluminum in man. In: HJ Gitelman (ed). Aluminum and Health. Marcel Decker Pub, NY: 101–124, 1989

    Google Scholar 

  2. Martyn CN, Osmond C, Edwardson JA, Baker DJP, Harris EC, Lacey RE: Geographical relation between Alzheimer's disease and aluminum in drinking water. Lancet 1: 59–62, 1989

    Google Scholar 

  3. Crapper DR, Quittkat S, Krishnan SS, Dalton AJ, DeBoni U: Intracellular aluminum content in Alzheimer's disease, dialysis encephalopathy and experimental aluminum encephalopathy. Acta Neuropathology 50: 19–24, 1980

    Google Scholar 

  4. Karlik SJ, Eichorn GL, Lewis PN, Crapper DR: Interaction of aluminum species with DNA. Biochemistry 19: 5991–5998, 1980

    Google Scholar 

  5. DeBoni U, Seger M, Crapper DR: Functional consequences of chromatin bound aluminum in cultured human cells. Neurotoxicology 1: 65–81, 1980

    Google Scholar 

  6. Sanderson CL, Crapper DR, DeBoni U: Altered steroid-induced puffing by chromatin bound aluminum, Can J Genet Cytol 24: 27–36, 1982

    Google Scholar 

  7. Crapper DR, Vin Dam T, Farnell BJ, Lewis PN: Aluminum inhibition of ADP-ribosylation in vivo and in vitro. Neurobehavioural Toxicol Teratol 5: 645–647, 1983

    Google Scholar 

  8. Lukiw WJ, Kruck TPA, Crapper DR: Alterations in human linker histone-DNA binding in the presence of aluminum salts in vitro and in Alzheimer's disease. Neurotoxicology 8: 291–302, 1987

    Google Scholar 

  9. Jagannatha Rao KS, Rao BS, Vishnuvardhan D, Prasad KVS: Alterations in the superhelical state of DNA by aluminum. Biochem Biophys Acta 1172: 17–20, 1993

    Google Scholar 

  10. Crapper DR, Dalton AJ: Aluminum induced neurofibrillary degeneration, brain electrical activity and alterations in acquisition and retention. Physiol Behav 10: 925–933, 1973

    Google Scholar 

  11. Jope RS: Aluminum toxicity-transport and sites of action. Neurobiol Aging 7: 525–532, 1986

    Google Scholar 

  12. Gutteridge JMC, Quinlan GJ, Clark I, Halliwell B: Aluminum salts accelerate peroxidation of membrane lipids stimulated by iron salts. Biochem Biophys Acta 835: 441–447, 1985

    Google Scholar 

  13. Jagannatha Rao KS: Effects of aluminum salts on synaptosomal enzymes – an in vitro kinetic study. Biochemistry International 28: 725–734, 1990

    Google Scholar 

  14. Jagannatha Rao KS: Effects of aluminum on the brain cells of rat. Biochem Int 28: 51057, 1992

    Google Scholar 

  15. Vierstra R, Haug A: The effect of Al on the physical properties of membrane lipids in Thermoplasama acidophilum. Biochem Biophys Res Commun 84: 138–143, 1978

    Google Scholar 

  16. Shi B, Haug A: Aluminum transport across neuroblastoma cells. J Neurochem 55: 551–558, 1990

    Google Scholar 

  17. Messenguy F: Regulation of arginase biosynthesis in S. cerevisae: isolation of a cis-dominant constitutive mutant for ornithine carbamoyltransferase synthesis. J Bacteriol 128: 49–55, 1976

    Google Scholar 

  18. Martin BR: The chemistry of aluminum as related to biology and medicine. Clinical Chem 32: 1797–1806, 1986

    Google Scholar 

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Jagannatha Rao, K., Easwaran, K. 27Al-NMR studies of aluminum transport across yeast cell membranes. Mol Cell Biochem 175, 59–63 (1997). https://doi.org/10.1023/A:1006885228074

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