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Palmitate incorporation into lipids pools of contracting red and white muscles

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Abstract

We compared the incorporation of the blood-borne [14C]-palmitate into selected lipid and phospholipid pools in rat muscles (soleus, red and white gastrocnemius), at rest and during contractions (15 and 60 tetani/min) in one leg (5 min) while the contralateral leg served as a control. [1-14C]-palmitate (20 µCi/rat) was administered into the carotid artery (t = 1 min). [14C]-palmitate deposition was greatest in soleus (100%) and lower in red (82%) and white gastrocnemius muscles (63%), respectively (p < 0.05). [14C] was deposited primarily into the tri-acylglycerol (∼50%) and phospholipid pools (∼30%) of soleus and red gastrocnemius muscles, and into the di-acylglycerol (∼30%), tri-acylglycerol (∼30%) and phospholipid pools (∼30%) in white gastrocnemius muscle. During contraction the concentrations of tri-acylglycerol were not changed. But, contraction increased [14C]-palmitate incorporation into soleus and red gastrocnemius muscles (600-700%) and into white gastrocnemius muscles (200%). Slightly more [14C] was directed from the phospholipids into the tri-acylglycerol pool during contraction. [14C]-palmitate deposition was also increased in the subclasses of phospholipids during contraction in red and white gastrocnemius. In conclusion, the deposition of [14C]palmitate into different lipid and phospholipid pools is quite rapid, and is dependent on contraction and the muscle fiber type. (Mol Cell Biochem 166: 73-83, 1997)

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Authors and Affiliations

  1. Department of Physiology, Medical Academy, 15-230, Bialystok, Poland

    Jan Gorski

  2. Department of Kinesiology, University of Waterloo, Waterloo, Ontario, N2L 3GI, Canada

    Arend Bonen

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  1. Jan Gorski
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  2. Arend Bonen
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Gorski, J., Bonen, A. Palmitate incorporation into lipids pools of contracting red and white muscles. Mol Cell Biochem 166, 73–83 (1997). https://doi.org/10.1023/A:1006882515938

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