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Inhibition of human lymphocyte function by organic solvents

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Abstract

We studied the direct effect of reactive hydroxyl precursors and inhibitors on CD4+ T-cell function. We used hydrogen peroxide plus ferrous chloride as the hydroxyl radical-generating system and di-methyl sulphourea, di-methyl sulfoxide, pyrrolidine dithiocarbonate, methanol, and ethanol, at a noncytotoxic concentration, as inhibitors. The immune parameter studies were proliferation and interleukin-2 production by peripheral blood lymphocytes stimulated with anti-CD3 antibody, phytohemagglutinin and alloantigens; proliferation, interleukin-2 production and mRNA expression of interleukin-4 and interferon gamma by allogeneic CD4+ T-cell clones stimulated with alloantigens. The results show that lymphocytes produce significant amounts of reactive oxygen species as measured by malondialdehyde produced in cultures. The hydroxyl radical-generating system did not change any of the cellular responses studied although it doubled Malondialdehyde production. Hydroxyl radical scavengers signi tly inhibited all responses at doses that didn't significantly decrease malondialdehyde production. DNA analysis failed to show evidence for apoptosis. Conclusion: Hydroxyl radical scavengers inhibit lymphocyte mitogenesis by a process that is independent of scavenging hydroxyl radicals.

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Authors and Affiliations

  1. Department of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W8, Canada

    Ahmed S. Shoker & Mohamed A. Murabit

  2. Department of National Research Council, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W8, Canada

    Fawsy F. Georges

  3. Department of Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W8, Canada

    Louis F. Qualtiere & Harry G. Deneer

  4. Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W8, Canada

    Kailash Prasad

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  1. Ahmed S. Shoker
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Shoker, A.S., Murabit, M.A., Georges, F.F. et al. Inhibition of human lymphocyte function by organic solvents. Mol Cell Biochem 171, 49–58 (1997). https://doi.org/10.1023/A:1006882114285

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