Abstract
The effect of intraventricular (IVT) administration of GABAA receptor agonist muscimol and GABAB receptor agonist, baclofen was examined on the activity of acetylcholinesterase (AChE), monoamine oxidase (MAO) and Na+, K+-ATPase in discrete areas of brain from estrogen-progesterone primed ovariectomized rats. AChE enzyme activity was increased in two subcellular fractions (soluble and total particulate) studied, with statistically significant changes in cerebral hemispheres (CH), cerebellum (CB), thalamus (TH) and hypothalamus (HT), Na+, K+-ATPase enzyme activity was decreased in both these fractions. MAO activity increased significantly in CH, TH and HT. The presented results suggest a functional relationship between GABAergic (inhibitory), cholinergic and monoaminergic (excitatory) systems by affecting the rate of degradation of the excitatory neurotransmitters and Na+, K+-ATPase. (Mol Cell Biochem 167: 107-111, 1997)
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Akema T, Kimura F: Differential effects of GABAA and GABAB receptor agonists on NMDA-induced and noradrenaline-induced luteinizing hormone release in the ovariectomized estrogen-primed rat. Neuroendocrinology 57: 28–33, 1993
Nissen R, Renaud LP: GABA receptor mediation of median preoptic nucleus-evoked inhibition of supraoptic neurosecretory neurons in rat. J Physiol 479: 207–216, 1994
Adler BA, Crowley WR: Evidence of gamma-aminobutyric acid modulation of ovarian hormonal effects on luteninizing hormone secretion and hypothalamic catecholamine activity. Endocrinology 118: 91–97, 1986
Akema T, Chiba A, Kimura F: On the relationship between noradrenergic stimulatory and GABAergic inhibitory systems in the control of luteinizing hormone secretion in female rat. Neuroendocrinology 52: 566–572, 1990
Kaur G, Kaur K: Effect of acute starvation on monoamine oxidase and Na+, K+-ATPase activity in rat brain. Mol Chem Neuropathol 13: 175–183, 1990
Paxinos G, Watson CRR, Emson P: AChE stained horizontal sections of rat brain in stereotaxic coordinates. J Neurosci Meth 3: 129–149, 1980
Ellman GL, Courtney KD, Andres V, Featherstone RM: A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Biopharmacol 7: 88–95, 1961
Dubois KP, Potter VR: The assay of animal tissues for respiratory enzyme. III. Adenosine triphosphatase. J Biol Chem 150: 185–187, 1943
McMurray WC, Begg RW: Effect of valinomycin on oxidative phosphorylation. Arch Biochem Biophys 84: 546–548, 1959
Fiske CH, Subbarow Y: The colorimetric determination of phosphorus. J Biol Chem 66: 375–381, 1925
Catravas GN, Takenaga J, McHale CG: Effect of chronic administration of morphine on monoamine oxidase activity in discrete regions of the brain of rats. Biochem Pharmacol 26: 211–214, 1977
Mayanil CSK, Kazmi SMI, Baquer NZ: Changes in monoamine oxidase activity in rat brain during alloxan diabetes. J Neurochem 38: 179–183, 1982
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951
Demling J, Fuchs E, Baumert M, Wuttke, W: Preoptic catecholamine. GABA and glutamate release in ovariectomized and ovariectomized estrogen-primed rats utilizing a push-pull cannula technique. Neuroendocrinology 41: 212–218, 1985
DeBoer P, Westerlink BHC: GABAergic modulation of striatal cholinergic interneurons: An in vivo microdialysis study. J Neurochem 62: 70–75, 1994
Decavel C, Vanden Pol AN: GABA: A dominant neurotransmitter in the hypothalamus. J Comp Neurol 302: 1019–1037, 1990
Rye DB, Wainer BH, Mesulam MM, Mulson EJ, Saper CB: Cortical projection arising from the basal forebrain: A study of cholinergic and non cholinergic components employing combined retrograde tracing and immunohistochemical localization of choline acetyltransferase. Neuroscience 13: 627–643, 1984
Sakai KY, Yoshimoto PH, Luppi P, Fort M, Mansari EI, Salvert D, Jouvet M: Lower brainstem afferents to the cat posterior hypothalamus: A double-labelling study. Brain Res Bull 24: 437–455, 1990b
Scatton B, Branimir Z, Jaroslov D, Kenneth GL, Jean C, Rene T, Bartholini G: GABA receptor stimulation: Effect of progabide SL (76002) on norepinephrine, dopamine, 5-HT turnover in rat brain areas: J Phar Exp Ther 220: 678–688, 1982
Gritti I, Mainville L, Jones BE: Projections of GABAergic and cholinergic basal forebrain and GABAergic preoptic-anterior hypothalamic neurons to the posterior lateral hypothalamus of the rat. J Comp Neurol 339: 251–268, 1994
Seong JY, Jarry H, Kuhnemuth S, Leonhardt S, Wuttke W, Kyungjin K: Effect of GABAergic compounds on gonadotropin releasing hormone receptor gene expression in the rat. Endocrinology 136: 2587–2593, 1995
Fuxe K, Andersson K, Ogren SO, Perez De La, Mora M, Schwarcz R, Hokfelt T, Eneroth P, Gustafsson JA, Skett P: GABA neurons and their interaction with monoamine neurons. In: GABA neurotrans-mitters. Alfred Benzon Symposium 12. Munksgaard, Copenhagen, 1978, pp 74–94
Jennes L, Stumpf WE, Tappaz ML: Anatomical relationships of dopaminergic and GABAergic systems with the GnRH-systems in the septo-hypothalamic area. Exp Brain Res 50: 91–99, 1983
Calabresi P, Stefani A, Mercuri NB, Bernardi G: Acetylcholine-dopamine balance in striatum: Is it still a target for antiparkinsonian therapy? In: M Frotscher U Misgeld (eds). Central Cholinergic Synaptic Transmission. Basel, Birkhauser Verlag, 1989, pp 315–321
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Kaur, G., Sharma, P., Bhardwaj, S. et al. GABA agonists and neurotransmitters metabolizing enzymes in steroid-primed OVX rats. Mol Cell Biochem 167, 107–111 (1997). https://doi.org/10.1023/A:1006876718783
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DOI: https://doi.org/10.1023/A:1006876718783