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Intermolecular contact sites in protein kinase CK2

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Abstract

Chemical crosslinking and analysis of CNBr-digested fusion products by immunoblotting with sequence-specific antibodies identifies an interaction between positions 55-70 of subunit β (β55-70) and 65-80 of subunit α (α65-80). This has been supported by crosslinking of subunits with peptides α65-80 and β55-70, by binding of subunits to immobilized peptides, and by the hindrance of coprecipitation with peptide-raised antibodies (anti-α65-80; anti-β55-70). Functionally, β55-70 is a negative regulatory region for the kinase activity of subunit α. The opposite, stimulatory property of subunit β has been assigned to its C-terminal part. Subdivision of peptide β155-181, that has stimulatory effect, into overlapping peptides and assaying for a binding and binding competition revealed a tight physical contact at β162-175. This region, however, is non-stimulatory indicating binding a necessary but not sufficient quality for stimulation. A contact might exist to regions surrounding C147 and/or C220 at subunit a as indicated by crosslinking and peptide competition. The crosslinking data also confirm a β-β contact in CK2 holoenzyme. Effects by non-ionic detergents show hydrophobic interactions to play an important role in catalytic activity adjustment.

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  1. Biochemische Zellphysiologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Andreas Krehan & Walter Pyerin

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  1. Andreas Krehan
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  2. Walter Pyerin
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Krehan, A., Pyerin, W. Intermolecular contact sites in protein kinase CK2. Mol Cell Biochem 191, 21–28 (1999). https://doi.org/10.1023/A:1006861102599

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