Skip to main content
SpringerLink
Log in

Potential sensitivity of hepatic specific protein regucalcin as a marker of chronic liver injury

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

A novel calcium-binding protein regucalcin has been shown to be specifically expressed in the liver of various specifies including human. Regucalcin concentration in the serum of patients with chronic liver injury was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. Serum samples were obtained from 42 persons who were diagnosed as liver disorder. Serum regucalcin concentration in all patients was in the range of 3.7-69.6 ng/ml, although regucalcin was not entirely seen in the serum of normal subjects (10 persons) without hepatitis. Meanwhile, in 18 patients with liver injury, serum glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities were normal value (less than 40 I.U./l). Serum GOT and GPT activities from 24 patients showed a comparatively higher level (50-234 I.U./l). The present results demonstrate the potential sensitivity of regucalcin as a marker of chronic liver injury. (Mol Cell Biochem 167: 187-190, 1997)

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Williamson JR, Cooper RK, Hoek JB: Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta 639: 243–295, 1981

    Google Scholar 

  2. Reinhart PH, Taylor WM, Bygrave FL: The role of calcium ions in the mechanisms of action of α-adrenergic agonists in rat liver. Biochem J 223: 1–13, 1984

    Google Scholar 

  3. Cheung WY: Calmodulin plays a pivotal role in cellular regulation. Science 202: 19–27, 1980

    Google Scholar 

  4. Nishizuka Y: Studies and perspectives of protein kinase C. Science 233: 305–312, 1986

    Google Scholar 

  5. Yamaguchi M: A novel Ca2+-binding protein regucalcin and calcium inhibition: Regulatory role in liver cell function. In: K Kohama (ed). Calcium Inhibition. Japan Sci Soc Press, Tokyo and CRC Press, Boca Raton, 1992, pp 19–41

    Google Scholar 

  6. Mori S, Yamaguchi M: Hepatic calcium-binding protein regucalcin decreases Ca2+/calmodulin-dependent protein kinase activity in rat liver cytosol. Chem Pharm Bull 38: 2216–2218, 1990

    Google Scholar 

  7. Yamaguchi M, Tai H: Inhibitory effect of calcium-binding protein regucalcin on Ca2+/calmodulin-dependent cyclic nucleotide phosphodi-esterase activity in rat liver cytosol. Mol Cell Biochem 106: 25–30, 1991

    Google Scholar 

  8. Yamaguchi M, Mori S: Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol 43: 140–146, 1990

    Google Scholar 

  9. Shimokawa N, Matsuda Y, Yamaguchi M: Genomic cloning and chro-mosomal assignment of rat regucalcin gene. Mol Cell Biochem 151: 157–163, 1995

    Google Scholar 

  10. Shimokawa N, Isogai M, Yamaguchi M: Specific species and tissue differences for the gene expression of calcium-binding protein regucalcin. Mol Cell Biochem 143: 67–71, 1995

    Google Scholar 

  11. Yamaguchi M, Isogai M: Tissue concentration of calcium binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay. Mol Cell Biochem 122: 65–68, 1993

    Google Scholar 

  12. Shimokawa N, Yamaguchi M: Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. FEBS Lett 305: 151–154, 1992

    Google Scholar 

  13. Yamaguchi M, Kanayama Y, Shimokawa N: Expression of calcium-binding protein regucalcin mRNA in rat liver is stimulated by calcitonin: the hormonal effect is mediated through calcium. Mol Cell Biochem 136: 43–48, 1994

    Google Scholar 

  14. Yamaguchi M, Oishi K, Isogai M: Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: Involvement of glucose, insulin and calcium as stimulating factors. Mol Cell Biochem 142: 35–41, 1995

    Google Scholar 

  15. Isogai M, Yamaguchi M: Calcium administration increases calcium-binding protein regucalcin concentration in the liver of rats. Mol Cell Biochem 143: 53–58, 1995

    Google Scholar 

  16. Isogai M, Shimokawa N, Yamaguchi M: Hepatic calciumbinding protein regucalcin is released into the serum of rats administered orally carbon tetrachloride. Mol Cell Biochem 131: 173–179, 1994

    Google Scholar 

  17. Isogai M, Oishi K, Yamaguchi M: Serum release of hepatic calcium-binding protein regucalcin by liver injury with galactosamine administration in rats. Mol Cell Biochem 136: 85–90, 1994

    Google Scholar 

  18. Yamaguchi M, Yamamoto T: Purification of calcium binding substance from soluble fraction of normal rat liver. Chem Pharm Bull 26: 1915–1918, 1978

    Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall E: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–273, 1951

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City, 422, Japan

    Masayoshi Yamaguchi

  2. Tokai Research Laboratories, Daiichi Pure Chemicals Company Limited, Tokaimura, Ibaraki, 319-11, Japan

    Mitsutaka Isogai & Noriaki Shimada

Authors
  1. Masayoshi Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mitsutaka Isogai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Noriaki Shimada
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamaguchi, M., Isogai, M. & Shimada, N. Potential sensitivity of hepatic specific protein regucalcin as a marker of chronic liver injury. Mol Cell Biochem 167, 187–190 (1997). https://doi.org/10.1023/A:1006859121897

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006859121897

Search

Navigation