Abstract
Nuclease susceptibility of the satellite DNA-containing chromatin of the liver of young (18 ± 2 weeks) and old (100 ± 5 weeks) rats was analysed using nick-translated rat 185 bp satellite I DNA fragment cloned in pBR322. With increasing concentration of DNaseI and micrococcal nuclease (MNase), multimeric forms of the satellite ladder gradually disappear in both the ages. The rate of disappearance is faster in young rats as compared to old ones. Such age-dependent decrease in the susceptibility of satellite DNA-containing chromatin reflects its condensation towards heterochromatization in old age.
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References
Noll M: Subunit structure of chromatin. Nature 251: 249–251, 1974
Gross DS, Garrard WT: Nuclease hypersensitive sites in chromatin. Ann Rev Biochem 57: 159–197, 1988
Singh S, Kanungo MS: DNase I hypersensitive sites of the 5′ region of the fibronectin gene of the liver of the rat. Biochem Biophys Res Commun 181: 131–137, 1991
Weintraub H, Groudine M: Chromosomal subunits in active genes have an altered conformation. Science 193: 848–856, 1976
Kornberg RD, Lorch Y: Chromatin structure and transcription. Ann Rev Cell Biol 8: 563–587, 1992
Tas S, Walford RL: Influence of disulfide reducing agents on fraction of the chromatin complex by endogenous nucleases and deoxyribonu-clease I in ageing mice. J Gerontol 37: 673–679, 1982
Berkowitz EM, Sanborn AC, Vaughan DW: Chromatin structure in neuronal and neuroglial cell nuclei as a function of age. J Neurochem 41: 523–567, 1983
Chaturvedi MM, Kanungo MS: Analysis of conformation of the chromatin of the brain of young and old rats. Molec Biol Rep 10: 215–219, 1985
Venugopal J, SubbaRao K: Gene expression in different cell types of ageing rat brain. J Neurochem 56: 812–817, 1991
Burgoyne LA, Hewish DR: In: H Busch (ed). The Cell Nucleus IV: Academic Press, New York, 1978, pp 48–73
Southern EM: Base sequence and evolution of guinea-pig satellite DNA. Nature 227: 794–798, 1970
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: In: Short Protocols in Molecular Biology (3rd ed). John Wiley & Sons, Inc. USA, 1995
Richmond TJ, Finch JT, Rushton B, Rhodes D, Klug A: Structure of the nucleosome core particle at 7A 0 resolution. Nature 311: 532–537, 1984
Sun YL, Yuan ZX, Bellard M, Chambon P: Digestion of the chicken b-globin gene chromatin with micrococcal nuclease reveals the presence of an altered nucleosomal array characterized by a typical ladder of DNA fragments. EMBO J 5: 293–300, 1986
van Holde KE: In: Chromatin: Springer-Verlag, New York, pp 355–408, 1989
Thakur MK, Kanungo MS: Interaction of histones and non-histone chromosomal proteins of the brain of new born and adult rats with deoxyribonucleic acid. Ind J Biochem Biophys 17: 29–34, 1980
Cartwright IL, Elgin SCR: Nucleosomal instability and induction of new upstream protein-DNA associations accompany activation of four small heat shock protein genes in Drosophila melanogaster. Mol Cell Biol 6: 779–791, 1986
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Thakur, M.K., Chaurasia, P. Nuclease susceptibility of the rat liver satellite DNA-containing chromatin decreases with age. Mol Cell Biochem 171, 45–48 (1997). https://doi.org/10.1023/A:1006850701989
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DOI: https://doi.org/10.1023/A:1006850701989