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Identification of proteins that associate with protein kinase CK2

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Abstract

In order to aid in an understanding of the cellular functions of protein kinase CK2, a search for interacting proteins was carried out using a 32P-labeled CK2 overlay method. Several proteins were found to associate with CK2 by this assay; among them, one protein of 110 kDa appeared to be the most prominent one. The possible association of CK2 with p110 was suggested by experiments involving the co-immunoprecipitation using anti-CK2 antibodies. Further analysis using GST-CK2 fusion proteins demonstrated that the CK2-p110 interaction occurred through the CK2α/α′ subunits. To identify p110, it was purified using a GST-CK2 affinity column, and internal amino acid sequencing was then performed. p110 was found to be nucleolin, a nucleolar protein that may be important for rRNA synthesis; a possible role of CK2 in the control of this process is suggested. Using the same CK2 overlay technique, another interacting protein, insulin receptor substrate 1 (IRS-1), was also identified. By applying a modified overlay method using individual 35S-labeled CK2 subunits, obtained by in vitro translation in rabbit reticulate lysates, it was determined that CK2 associates with IRS-1 through its α/α′ subunits; i.e. in keeping with the fact that IRS-1 is a known substrate for CK2. However, further work is needed to examine the association of CK2 with IRS-1 in vivo in order to fully understand the significance of the interaction.

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Authors and Affiliations

  1. Department of Pharmacology and Biochemistry, University of Washington, Seattle, WA, USA

    Dongxia Li, Grazyna Dobrowolska & Edwin G. Krebs

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  1. Dongxia Li
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  2. Grazyna Dobrowolska
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  3. Edwin G. Krebs
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Li, D., Dobrowolska, G. & Krebs, E.G. Identification of proteins that associate with protein kinase CK2. Mol Cell Biochem 191, 223–228 (1999). https://doi.org/10.1023/A:1006848515861

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