Abstract
Lithostathine may play a physiological role in preventing the precipitation of excess calcium in the pancreatic juice. The hypothesis has been advanced that in chronic calcifying pancreatitis the abnormal biosynthesis of lithostathine might be the original defect to which genetic proneness to the disease may be ascribed. The aim of the present work was to study lithostathine messenger RNA expression in the pancreas of patients with different types of pancreatitis. Lithostathine and chymotrypsinogen mRNA were determined in surgical specimens obtained from the pancreases of the following subjects: (a) 13 patients with chronic alcoholic pancreatitis (84.6% calcified); (b) 4 patients with chronic hereditary pancreatitis (all calcified); (c) 6 patients with chronic obstructive pancreatitis (4 calcified); and (d) 27 subjects suffering from pancreatic cancer. Significantly lower concentrations of both mRNAs were found in the pancreases of chronic pancreatitis patients than in non-cancerous tissue from pancreatic cancer subjects. However, about 70% of the pancreatic cancer subjects showed lithostathine and chymotrypsinogen mRNA levels comparable to those of chronic pancreatitis patients. These results indicate that the decrease in the level of mRNA is not specific to lithostathine and it is unrelated to the presence of pancreatic stones.
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References
Bernard JP, Adrich Z, Montalto G, Multigner L, Dagorn JC, Sarles H, De Caro A: Immunoreactive forms of pancreatic stone protein in six mammalian species. Pancreas 6: 162–167, 1991
De Caro A, Lohse J, Sarles H: Characterization of a protein isolated from pancreatic calculi of men suffering from chronic calcifying pancreatitis. Biochem Biophys Res Commun 87: 1176–1182, 1979
Bernard JP, Adrich Z, Montalto G, De Caro A, De Reggi M, Sarles H, Dagorn JC: Inhibition of nucleation and crystal growth of calcium carbonate by human Lithostathine. Gastroenterology 103: 1277–1284, 1992
Bimmler D, Graf R, Scheele GA, Frick TW: Pancreatic stone protein (Lithostathine), a physiologically relevant pancreatic calcium carbonate crystal inhibitor? J Biol Chem 272: 3073–3082, 1997
Sarles H, Bernard JP, Johnson C, Chir M: Pathogenesis and epidemiology of chronic pancreatitis. Ann Rev Med 40: 453–468, 1989
Sarles H, Bernard JP: Lithostathine and pancreatic lithogenesis. Gastroenterol Intl 4: 130–134, 1991
Provansal-Cheylan M, Mariani A, Bernard JP, Sarles H, Dupuy P: Pancreatic stone protein: quantification in pancreatic juice by enzymelinked immunosorbent assay and comparison with other methods. Pancreas 4: 680–689, 1989
Schmiegel W, Buchert M, Kalthoff H, Roeder C, Butzow G, Grimm H, Kremer B, Soehendra N, Schreiber H-W, Thiele H-G, Greten H: Immunochemical characterization and quantitative distribution of pancreatic stone protein in sera and pancreatic secretions in pancreatic disorders. Gastroenterology 99: 1421–1430, 1990
Giorgi D, Bernard JP, Rouquier S, Iovanna J, Sarles H, Dagorn JC: Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis. J Clin Invest 84: 100–106, 1989
Chirgwin JM, Przybyla AK, MacDonald RJ, Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Am Chem Soc 18: 5294–5299, 1979
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning. Cold Spring Harbor Laboratory Press, 1989
White BA, Carter Bancroft F: Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. J Biol Chem 257: 8569–8572, 1982
Sarles H: Etiopathogenesis and definition of chronic pancreatitis. Dig Dis Sci 31: 91S–107S, 1986
Pap A, Boros L: Alcohol-induced chronic pancreatitis in rats after temporary occlusion of biliopancreatic ducts with ethibloc. Pancreas 4: 249–255, 1989
Tanaka T, Ichiba Y, Miura Y, Itoh H, Dohi K: Pathogenesis of chronic alcoholic pancreatitis. Am J Gastroenterol 85: 1536, 1990
Konishi K, Izumi R, Kato O, Yamaguchi A, Miyazaki I: Experimental pancreatolithiasis in the dog. Surgery 89: 687–691, 1987
Sakakibara A, Okumura N, Hayakawa T, Kanzaki M: Ultrastructural changes in the exocrine pancreas of experimental pancreatolithiasis in dogs. Am J Gastroent 77: 498–503, 1982
Cavallini G: Is chronic pancreatitis a primary disease of the pancreatic ducts? A new pathogenetic hypothesis. Ital J Gastroenterol 25: 391–396, 1993
Cavallini G, Bovo P, Filippini M, Vaona B, Frulloni L, Di Francesco V, Rigo L, Brunori MP, Ferrarini M, Andreaus MC, Tebaldi M, Angelini G, Procacci C, Pederzoli P: Chronic obstructive pancreatitis (COP) is a lithiasic disease. Pancreas 8: 751, 1993
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Cavallini, G., Bovo, P., Bianchini, E. et al. Lithostathine messenger RNA expression in different types of chronic pancreatitis. Mol Cell Biochem 185, 147–152 (1998). https://doi.org/10.1023/A:1006843309128
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DOI: https://doi.org/10.1023/A:1006843309128