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Effects of tetramethylpyrazine on the release of PGI2 and TXA2 in the hypoxic isolated rat heart

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We examined the effect on 6-Keto-PGF1a and TXB2 (the stable metabolites of PGI2 and TXA2) outflow of pretreatment with tetramethylpyrazine in the hypoxic isolated rat heart. In control hearts, 6-Keto-PGF1a was increased from 185 ± 46 pg/ml of baseline value to 335 ± 76 pg/ml after 2 min of hypoxia and TXB2 increased from 136 ± 28 pg/ml of baseline value to 230 ± 43 pg/ml at 20 min of hypoxia and 252 ± 32 pg/ml after 5 min of reoxygenation. Pretreatment with tetramethylpyrazine increased the 6-Keto-PGF1a concentration to 266 ± 51 pg/ml (143% of control heart), 471 ± 89 pg/ml (150% of control heart) and 332 ± 47 pg/ml (195% of control heart) at 15 min of normoxia, 2 min of hypoxia and 5 min of reoxygenation, respectively (p < 0.05 vs control). On the other hand, tetramethylpyrazine diminished the release of TXB2 to 78 ± 21 pg/ml (174% of control heart), 160 ± 30 pg/ml (144% of control heart), and 196 ± 23 pg/ml (128% of control heart) at 15 min of normoxia, 20 min of hypoxia and 5 min of reoxygenation, respectively (p < 0.05 vs control). These data show that pretreatment with tetramethylpyrazine enhances PGI2 outflow and attenuates release of TXA2 in the rat heart during normoxia, hypoxia and reoxygenation. (Mol Cell Biochem 167: 153-158, 1997)

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Research Centre, Hôpital du Sacré-Coeur de Montréal, Université de Montréal, Montréal, Québec, Canada

    Jun Feng

  2. Department of Cardiology, First Teaching Hospital, Henan Medical University, Zhengzhou, Peoples Republic of China

    Ruiyuan Liu

  3. Department of Thoracic and Cardiovascular Surgery, First Teaching Hospital, Henan Medical University, Zhengzhou, Peoples Republic of China

    Guozhen Wu

  4. Department of Physiology, First Teaching Hospital, Henan Medical University, Zhengzhou, Peoples Republic of China

    Shuben Tang

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  1. Jun Feng
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Feng, J., Liu, R., Wu, G. et al. Effects of tetramethylpyrazine on the release of PGI2 and TXA2 in the hypoxic isolated rat heart. Mol Cell Biochem 167, 153–158 (1997). https://doi.org/10.1023/A:1006837606488

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