Abstract
Oxygen derived free radicals are known to play an important role in the etiology of tissue injury in rheumatoid arthritis. The effect of milk extract of Semecarpus anacardium nuts at the dose level of 150 mg/kg body weight for 14 days on adjuvant arthritis was studied for gaining insight into the intrigue disease in relation to the lipid peroxidation and antioxidant defence system. Increased lipid peroxides' levels in both plasma and tissues (liver, kidney and heart) of adjuvant arthritis was significantly decreased by the administration of the drug. The antioxidant defence system studied in tissues of arthritic animals were altered significantly as evidenced by the decreased level of non-enzymatic antioxidants (GSH, vitamin E, vitamin C, NPSH and TSH) and enzymatic antioxidants (catalase and GPx except SOD). Administration of Semecarpus anacardium nut extract brings back the altered antioxidant defence components to near normal levels. These observations suggest that the diseased stat e of adjuvant arthritis may be associated with augmented lipid peroxidation and the administration of the drug may exert its antiarthritic effect by retarding lipid peroxidation and causing a modulation in cellular antioxidant defence system. (Mol Cell Biochem 175: 65–69, 1997)
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Blake DR, Hall DA, Treby DA, Halliwell B, Gutteridge JMC: Protection against superoxide and hydrogen peroxide in synovial fluid from rheumatoid patients. Clin Sci 61: 483–486, 1981
Freeman BA, Crapo JD: Biology of disease, free radicals and tissues injury. Lab Invest 47: 412–426, 1982
Holoshitz J, Matitiau A, Cohen IR: Arthritis induced in rats by cloned T lymphocytes responsive to mycobacteria but not to collagen type II. J Clin Invest 73: 211–215, 1984
Robinson SL, Cotran SC, Kumar V: In: Pathologic Basic of Disease. W.B. Saunders Company, 3rd edn, 1984, p 1347
Lunec J, Halloran SP, White AG, Dormandy TL: Free-radical oxidation (peroxidation) products in serum and synovial fluid in rheumatoid arthritis. J Rheumatol 8: 233–234, 1981
Babior BM, Kipnes RS, Curnutte JT: Biological defense mechanism: The production by leucocytes of superoxide, a potent bactericidal agent. J Clin Invest 52: 741–744, 1973
Tisdale MJ, Mohmoud MB: Activities of free radical metabolising enzymes in tumours. Br J Cancer 47: 809–812, 1983
Barber DA, Harris SR: Oxygen free radicals and antioxidants: a review. Am Pharm 34 (Sup): 26–35, 1994
Kritikar KR, Basu BD: In: Indian medicinal plants. Vol. 1, Allahabad, Sudhindra Nath Basu, 1918, p 384
Nadkarni AK: Indian Materia Medica, 3rd edn, Bombay, Popular Book Depot Ltd., 1954, p 1119
Vijayalakshmi T, Muthulakshmi V, Sachdanandam P: Effect of milk extract of Semecarpus anacardium nut on adjuvant arthritis – A dose dependent study in Wistar albino rats. Gen Pharmacol 27: 1223–1226, 1996
Formulary of Siddha Medicine. Published by the Indian Medical Practitioners Co-operative Pharmacy and Stores Ltd., Madras, India, 2nd edn, 1972, p 197
Moron MS, Difieree JW, Mannervik KB: Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochem Biophys Acta 582: 67–68, 1979
Omaye ST, Turabull JD, Sanberlib HE: Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Meth Enzymol 62: 1–11, 1979
Sinha AK: Colorimetric assay of catalase. Anal Biochem 47: 380–590, 1973
Rotruk JT, Pope AL, Ganther HE: Selenium, biochemical role as a component of glutathione peroxidase purification and assay. Science 179: 588–590, 1973
Marklund S, Marklund G: Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469–471, 1974
Devasagayam TPA, Tarachand V: Decreased LPO in the rat kidney during gestation. Biochem Biophys Res Commn 145: 469–474, 1987
Desai ID: Vitamin E analysis methods for animal tissues. Meth Enzymol 105: 138–143, 1984
Yagi K: Simple fluorimetric assay for lipid peroxidation in blood plasma. Biochem Med 15: 212–215, 1976
Sedlak J, Lindsay RH: Estimation of total protein bound and nonprotein bound sulphydryl groups in the tissue with Ellman's reagent. Anal Biochem 25: 192–205, 1968
Van Eden W, Holoshitz J, Nevo Z, Frenkel A, Klatman A, Cohen IR: Arthritis induced by a T-lymphocytes clone that responds to Mycobacterium tuberculosis and to cartilage proteoglycans. Proc Natl Acad Sci USA 82: 5117–5120, 1985
Green Wald RA: Oxyradical and connective tissue J. Rheumatol 8: 185–187, 1981
Babior BM: Oxygen dependent microbial killing by phagocytes. N Eng J Med 298: 721–725, 1981
Spector WG: The acute inflammatory response. Ann NY Acad Sci 116: 747–752, 1964
Bardhan P, Sharma SK: Lipid peroxidation in experimental inflammation. Arogya – J Health Science IX: 120–124, 1983
Satyavathi GV, Prasad DN, Das PK, Singh HD: Antiinflammatory activity of Semecarpus anacardium Linn. A preliminary study. Ind J Physiol Pharmacol 13: 37–43, 1969
Klopman G, Dimayuga ML: Computer-automated structure evaluation of flavonoids and other structurally-related components as glyoxalase enzyme inhibitors. Mol Pharmacol 34: 218–222, 1988
Marklund SL, Kling PA, Nilsson S, Ohman M: Plasma extra cellular superoxide dismutase in the acute phase response induced by surgical trauma and inflammatory disorder. Scand J Clin Lab Invest 47: 567–570, 1987
Rister M, Banchner RL: The alteration of SOD, catalase, glutathione peroxides, NADPH, cyt C oxidase in guinea pig polymorphonuclear leucocytes and alveolar macrophage during hypoxia. J Clin Invest 58: 1174–1184, 1976
Parnham MJ, Graf E, Hoff E, Niemann R: Selenium hydroperoxides and atherosclerosis. Agents and Action 22: 3–4, 1987
Niemann R: Selenium Mottonen T, Hannonen P, Seppala O: Glutathione and selenium in rheumatoid arthritis. Clin Rheumatol 3: 195–200, 1984
Nicotera P, Orrenius S: Role of thiols in protection against biological reactive intermediates. Adv Exp Med Biol 187: 41–51, 1986
Meister A, Anderson S: Glutathione. Ann Rev Biochem 52: 711–760, 1983
Beck FJ, White house MW: Effect of adjuvant arthritis disease in rat in cyclophosphamide and isophosphamide metabolism. Biochem Pharmacol 22: 2453–2458, 1973
Kornburst DJ, Mavis RD: Relative susceptibility of microsomes from lung, heart, liver, kidney, brain, and testes to lipid peroxidation correlation with vitamin E content. Lipids 15: 315–322, 1980
Frer B, England L, Ames BN: Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA 86: 6377–81, 1989
Bors W, Heller W, Michel C, Saran M: Flavonoids as antioxidants: determination of radical-scavenging efficiencies. In: L Packer, AN Glazer (eds). Methods in Enzymology. Vol. 186, Academic Press, San Diego 1990, pp 343–355
Torel J, Cillard J, Cillard P: Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry 25: 383–385, 1986
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Vijayalakshmi, T., Muthulakshmi, V. & Sachdanandam, P. Salubrious effect of Semecarpus anacardium against lipid peroxidative changes in adjuvant arthritis studied in rats. Mol Cell Biochem 175, 65–69 (1997). https://doi.org/10.1023/A:1006837312145
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DOI: https://doi.org/10.1023/A:1006837312145