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Effect of Lipoprotein-X on lipid metabolism in rat kidney

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Abstract

Lipoprotein-X (Lp-X) is found in the plasma of patients with familiallecithin: cholesterol acyltransferase (LCAT) deficiency syndromes. Themajority of the patients with this disorder develop progressiveglomerulosclerosis. In this study, the effect of Lp-X on lipid metabolism inperfused rat kidney was investigated. Lp-X was isolated from plasma ofpatients with familial LCAT deficiency by sequential ultracentrifugation andgel filtration column chromatography. Rat kidneys were perfused for 1-2 hwith Krebs-Henseleit buffer containing 20 µM [1-14C]acetate or 20µM [Me-3H]choline. In the presence of Lp-X, no significant differencein the incorporation of radioactivity into triglycerides, cholesterol,phosphocholine, CDP-choline and sphingomyelin was observed. However,incorporation of radioactivity into cholesteryl esters andphosphatidylcholine was significantly elevated in Lp-X perfused kidneys. Thecontents of cholesterol, cholesteryl esters and phosphatidylcholine werealso significantly increased in Lp-X perfused kidneys. The increase in lipidcontent in the Lp-X perfused kidney is attributed to the direct depositionof Lp-X lipids into the organ. The increase in the labelling of cholesterylesters was attributed to the increase of available substrate (cholesterol)for the acyl-CoA:cholesterol acyltransferase (ACAT) reaction. The increasein phosphatidylcholine labelling was caused by a reduced turnover of thenewly synthesized labelled phosphatidylcholine during Lp-X perfusion.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of British Columbia, Atherosclerosis Specialty Laboratory, St. Paul's Hospital, 1081 Burrard Street, Vancouver, British Columbia, Canada

    May Ly, Ding Z. Fang & Jiri Frohlich

  2. Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Manitoba, Canada

    Patrick C. Choy

Authors
  1. O Karmin
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  2. May Ly
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  3. Ding Z. Fang
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  4. Jiri Frohlich
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Karmin, O., Ly, M., Fang, D.Z. et al. Effect of Lipoprotein-X on lipid metabolism in rat kidney. Mol Cell Biochem 173, 17–24 (1997). https://doi.org/10.1023/A:1006836704042

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