Abstract
The present study is a comparison of tRNA conformation from ovary of Heteropneustes fossilis in its active phase of reproduction (when it is highly engaged in protein synthesis i.e. previtellogenic phase) with inactive phase (when tRNA is mainly stored in mature ovary i.e. spawning phase). Transfer RNA of active phase is shown to be compact, flexible and susceptible towards nuclease. Compact tRNA structure is evidenced by higher hyperchromicity and presence of relatively less Gm modifications thereby allowing adequate hydrogen bonding between D loop and T loop. Higher sensitivity of tRNA towards Mg++ reflects its higher flexibility towards internal environment. This structure of tRNA may be required for active protein synthesis. On the other hand tRNA of inactive phase is shown to be relaxed but resistant towards nuclease which may be favoured for storage in mature ova of a teleost as maternal carry over.
References
Curtis D, Iehmann R & Zamore PD (1995) Cell 81: 171
Sampson J & Uhlenbeck O (1983) Proc. Natl. Acad. Sci. USA 85: 1033
Bïïttcher V, Senger B, Schumacher S, Reinbolt J & Fasiolo F (1994) Biochem and Biophys. Res. Commun. 200(1): 370
Nameki N, Asahara H, Shimizu M, Okada N & Himeno H (1995) Nucleic Acids Res. 23(3): 389
Draper DE (1996) Trends in Biochemical Science 21: 145
Caston JD (1971) Dev. Biol. 24: 19
Ueda T, Watanabe K & Ohta T (1983) Proc. Japan Acad. 59: 339
Cohen SS (1978) Nature 274: 209
Sarkar M, Subbarayan PR & Vinayak M (1994) Mol. Biol. Reports 20: 9
Subbarayan PR, Sarkar M & Vinayak M (1995) Mol. Biol. Reports 21: 113
Kumagai I, Watanabe K & Oshima T (1982) J. Biol. Chem. 257(13): 7388
Heyman T, Agoutin B, Fix C, Dirheimer G & Keith G (1994) FEBS Lett. 347: 143
Xue H, Glasser AL, Desgres J & Grosgean H (1993) Nucleic Acids Res. 21(10): 2479
Kowalak JA, Dalluge JJ, McCloskey JA & Stetter KO (1994) Biochemistry 33: 7869
Watanabe K, Oshima T, Iijima J, Yamaizumi Z & Nishimura S (1980) J. Biochem. 87: 1
Yokogawa T, Kumazawa Y, Miura IK & Watanabe K (1989) Nucleic Acids Res. 17(7): 2623
Ohyama T, Nishikawa K & Takemura S (1985) J. Biochem 97: 29
SreeNagesh MV (1995) Ph.D. Thesis, Indian Inst. Sci., Bangalore, India
Agris PF, Koh H & Söll D (1973) Arch. Biochem. Biophys. 154: 277
Wrede P, Wurst R, Vournakis J & Rich A (1979) J. Biol. Chem. 254: 9608
Ladner JE, Jack A, Robertus JD, Brown RS, Rhodes D, Clark BFC, Klug A (1975) Proc. Natl. Acad. Sci. USA 72: 4414
Quigley GJ, Wang AHJ, Seeman NC, Suddath FL, Rich A, Sussman JL & Kim SH (1975) Proc. Natl. Acad. Sci. USA 72: 4866
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sarkar, M., Vinayak, M. Comparison of tRNA conformation during different phases of reproduction. Mol Biol Rep 25, 127–133 (1998). https://doi.org/10.1023/A:1006836415897
Issue Date:
DOI: https://doi.org/10.1023/A:1006836415897