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Antigenic similarities to Paracoccidioides brasiliensis in thermo-dependent dimorphic fungi isolated from soil in Botucatu, SP, Brazil

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Abstract

We compared the antigenic characteristics of two thermo-dependent dimorphic fungi isolated from soil in Botucatu, an endemic area of paracoccidioidomycosis (PCM) and Paracoccidioides brasiliensis. The soil isolates grew as cerebriform colonies at 37 °C (yeast form) and as cottonous colonies at 25 °C (mycelial form). No pathogenicity for ddY mice or hamsters were observed. In immunodiffusion test, there were precipitation bands between the 2 soil isolates and pooled PCM patient sera. There were also common precipitation bands at 21, 50 and 58 kDa between the soil isolates antigens and PCM patient sera by Western-blotting, but no gp43 kDa band. No gene for gp 43 kDa protein was detected in the soil isolates by PCR. The fact that these isolates were obtained from an endemic area of PCM and there were some antigenic similarities between the soil isolates and P. brasiliensis in immunodiffusion test and Western-blotting may have some importance in epidemiological surveys done with paracoccidioidin as well interfering with the immune response of the exposed population.

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References

  1. Restrepo AM. The ecology of Paracoccidioides brasiliensis: a puzzle still unsolved. J Med Vet Mycol 1985; 23: 323–334.

    CAS  Google Scholar 

  2. San-Blas G. Paracoccidioidomycosis and its etiologic agent Paracoccidioides brasiliensis. J Med Vet Mycol 1993; 31: 99–113.

    PubMed  CAS  Google Scholar 

  3. Albornoz MB. Isolation of Paracoccidioides brasiliensis from rural soil in Venezuela. Sabouraudia 1971; 9: 248–253.

    PubMed  Google Scholar 

  4. Ferreira MS, Freitas C, Lacaz CS, Del Negro GMB, Melo NT, Garcia NM, Assia CM, Salebian A, Heins-Vaccari EM. Isolation and characterization of Paracoccidioides brasiliensis strain from a dog food probably contaminated with soil in Uberlandia, Brazil. J Med Vet Micol 1990; 28: 253–256.

    CAS  Google Scholar 

  5. Negroni P. El Paracoccidioides brasiliensis vive saprofiticamente en el suelo argentino. Prensa Med Argentina 1966; 53: 2831–2832.

    Google Scholar 

  6. Brummer E, Castaneda E, Restrepo A. Paracoccidioidomycosis: an update. Clin Microbiol Rev 1993; 6: 89–117.

    PubMed  CAS  Google Scholar 

  7. Naiff RD, Ferreira LCL, Barrett TV, Naiff MF, Arias JR. Paracoccidioidomicose enzó otica emtatus (Dasypus novemcinctus) no estado do Pará. Rev Inst Med S Paulo 1986; 79: 765–772.

    Google Scholar 

  8. Restrepo A. Actualizacibn sobre la paracoccidioidomicosis y su agente etiologico, 1986- 1989. Interciencia 1990; 15: 193–199.

    Google Scholar 

  9. Gezuele E. A islamiento de Paracoccidioides sp. de heces de pinguino de la Antartida, res. B2. Resumenes IV Encuentro Internacional sobre Paracoccidioidomicosis, Caracas, Venezuela, 1989.

  10. Grose, E. Paracoccidioides brasiliensis recovered from the intestinal tracts of three bats (Artibeus lituratus) in Colombia S.A. Sabouraudia 1965; 4: 124–125.

    PubMed  CAS  Google Scholar 

  11. Conti-Diaz I, Rilla FD. Hipotesis sobre el nicho ecologico de Paracoccidioides brasiliensis. Rev Med uruguay 1989; 5: 97–103.

    Google Scholar 

  12. Montenegro MR, Miyaji M, Franco M, Nishimura K, Coelho KIR, Hone Y, Mendes RP, Sano A, Fukushima K, Fecchio D. Isolation of fungi from nature in the region of Botucatu, SP, Brazil, an endemic area of Paracoccidioidomycosis. Memorias do Institute Oswaldo Cruz, 1996; 91: 665–670.

    CAS  Google Scholar 

  13. Marques SA, Franco MF, Mendes RP, Silva NCA, Baccili C, Curcelli ED, Feracin ACM, Oliveira CS, Taglianini JVL, Dillon NL, Aspectos epidemiológicos da paracoccidioidomicose na área endemica de Botucatu (São Paulo, Brasil). Rev Inst Med Trop S Paulo 1983; 25: 87–92.

    PubMed  CAS  Google Scholar 

  14. Freire-Maia DV, Garcia MG, Mendes RP, Marques SA, Curi PR, Souza LR. Chromosome aberrations in lymphocyte cultures from paracoccidioidomycosis patients. J Med Vet Mycol 1994; 32: 199–203.

    PubMed  CAS  Google Scholar 

  15. Sano A, Miyaji M, Nishimura K, Franco M. Studies on the relationship between the pathogenicity of Paracoccidioides brasiliensis in mice and its growth rate under different oxygen atmosphere. Mycopathologia 1990; 114: 93–101.

    Article  Google Scholar 

  16. Sano A, Kurita N, Coelho KIR, Takeo K, Nishimura K, Miyaji M. A comparative study of four different staining methods for estimation of live yeast form cells of Paracoccidioides brasiliensis. Mycopathologia. 1993; 124: 157–161.

    Article  PubMed  CAS  Google Scholar 

  17. Lowry OH, Rosebrough NH, Farr AL, Randall RJ. Protein measurement with the Folin phenol regent. J Biol Chem 1951; 193: 265–275.

    PubMed  CAS  Google Scholar 

  18. Towbin H, Staelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide to nitrocellulose sheets: Produce and some applications. Proc Natl Acad Sci USA, 1979; 76: 4350–4354.

    Article  PubMed  CAS  Google Scholar 

  19. Camargo ZP, Taborda CP, Rodorigues EG, Travassos LR. The use of cell-free antigens of Paracoccidioides brasiliensis in serological tests. J Med Vet Mycol 1991; 29: 31–38.

    PubMed  CAS  Google Scholar 

  20. Camargo ZP, Taborda CP. Antigenic relationship between Paracoccidioides brasiliensis isolated from feces of a penguin and human isolate of P. brasiliensis. J Med Vet Mycol 1993; 31: 347–352.

    Google Scholar 

  21. Cisalpino PS, Puccia R, Yamauchi LM, Cano MIN, da Silveira JF, Travassos LR. Cloning, characterization, and epitope expression of major diagnostic antigen of Paracoccidioides brasiliensis. J Biol Chem 1996; 271: 4553–4560.

    Article  PubMed  CAS  Google Scholar 

  22. Scapolio SHB, Montenegro MR, Franco M. Caracterizacon de fungos isolados de solo da area endemica de Botucatu. Similares ao Paracoccidioides brasiliensis. Resumens p. 172, VI. Encuentro Internacional sobre Paracoccidioidomicosis, Montevideo, Uruguay, 21 al 24 de Marzo de 1996.

  23. Franco M, Bagagli E, Cunha M, Chamma LG, Fecchio D. Paracoccidioides brasiliensis antigen baches from the same isolate show immunological and biochemical differences. Mycopathologia 1996; 135: 13–19.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Infections, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1, Chuo-ku, Chiba, 260, Japan

    Ayako Sano, Kazuko Nishimura, Kazutaka Fukushima & Makoto Miyaji

  2. Natural History Museum and Institute, Chiba, Japan

    Yoshikazu Horie

  3. Departamento de Anatomia e Patologia, EPM, Universidade Federal de São Paulo, UNIFESP, SP, Brazil

    Marcello Franco

  4. Departamento de Molestias Infecciosas e Parasitarias, Faculdade de Medicina, UNESP, Botucatu, SP, Brazil

    Rinaldo Poncio Mendes

  5. Departamento de Patologia, Faculdade de Medicina, UNESP, Botucatu, SP, Brazil

    Kunie Iabuki Rabello Coelho, Silvia Helena Barbin Scapolio & Mario Rubens Montenegro

  6. Disciplina de Biologia Celular, Universidade Federal de São Paulo, UNIFESP, SP, Brazil

    Zoilo Pires dè Camargo

Authors
  1. Ayako Sano
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  2. Kazuko Nishimura
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  3. Yoshikazu Horie
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  4. Marcello Franco
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  5. Rinaldo Poncio Mendes
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  6. Kunie Iabuki Rabello Coelho
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  7. Silvia Helena Barbin Scapolio
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  8. Kazutaka Fukushima
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  9. Zoilo Pires dè Camargo
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  10. Makoto Miyaji
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  11. Mario Rubens Montenegro
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Sano, A., Nishimura, K., Horie, Y. et al. Antigenic similarities to Paracoccidioides brasiliensis in thermo-dependent dimorphic fungi isolated from soil in Botucatu, SP, Brazil. Mycopathologia 138, 37–41 (1997). https://doi.org/10.1023/A:1006835903685

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  • DOI: https://doi.org/10.1023/A:1006835903685

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