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Contribution of topoisomerase I to conversion of single-strand into double-strand DNA breaks

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Abstract

An in vitro system composed of nicked pBR322 DNA and purified topoisomerase I was employed to study the efficiency of the topoisomerase I-driven single-strand to double-strand DNA breaks conversion. At 1.4 × 105 topoisomerase I activity units per mg DNA about 20% single-strand nicks were converted into double-strand breaks during 30 min due to topoisomerase I action. Camptothecin inhibited the conversion. The conversion was also inhibited when the relaxing activity of the used topoisomerase I was increased by phosphorylation of the enzyme with casein kinase 2. The presented data suggest that topoisomerase I may be involved in production of double-stranded breaks in irradiated cells and that this process positively depends on the amount of topoisomerase I but not on its phosphorylation state.

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Authors and Affiliations

  1. Institute of Biochemistry, Warsaw University, 02–089, Warsaw, Poland

    B. Kowalska-Loth, I. Bubko, B. Komorowska & K. Staron

  2. Institute of Nuclear Chemistry and Technology, Warsaw, Poland

    I. Szumiel

Authors
  1. B. Kowalska-Loth
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  2. I. Bubko
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  3. B. Komorowska
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  4. I. Szumiel
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  5. K. Staron
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Kowalska-Loth, B., Bubko, I., Komorowska, B. et al. Contribution of topoisomerase I to conversion of single-strand into double-strand DNA breaks. Mol Biol Rep 25, 21–26 (1998). https://doi.org/10.1023/A:1006831527609

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  • DOI: https://doi.org/10.1023/A:1006831527609

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