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Immunocytochemical detection of enhanced expression of c-myc protein in the heart of cardiomyopathic hamster

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Abstract

An immunocytochemical study was performed to examine the expression of cellular c-myc protein in the heart of 30-, 120- and 180-day-old cardiomyopathic Syrian UM-X7.1 hamsters. The heart of age- and sex-matched BIO-RB hamster was used as normal control. In paraffin sections, an immunostaining for c-myc was markedly increased in cytoplasm of cells from the UM-X7.1 heart as compared with that of the BIO-RB heart which showed a weak staining. However, c-myc was localized in nuclei of cells in frozen sections of the heart. Specific cell types of the heart were differentiated with anti-vimentin, and we found that the increased expression of c-myc was present in nuclei of muscle cells of the UM-X7.1 myocardium. A quantitative study of c-myc-positive nuclei of muscle and nonmuscle cells was carried out by a video micrometer. The mean number of c-myc-positive nuclei of muscle cells was significantly higher in the cardiomyopathic heart than in the control heart from hamsters of all ages studied. These results suggest that the increase of c-myc protein may relate to the pathological state or pathogenesis of the hereditary cardiomyopathy.

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Authors and Affiliations

  1. Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, 830, Japan

    Noboru Fujitani & Hiroshi Kimura

  2. School of Allied Health Sciences, Faculty of Medicine, Osaka University, Osaka, 560, Japan

    Naomasa Kawaguchi & Shunzo Onishi

  3. Department of Pathology, Saga Medical School, Saga, 849, Japan

    Shuji Toda

  4. Department of Biochemistry, Saga Medical School, Saga, 849, Japan

    Sueo Matsumura

Authors
  1. Noboru Fujitani
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  2. Naomasa Kawaguchi
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  3. Shuji Toda
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  4. Sueo Matsumura
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  5. Hiroshi Kimura
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  6. Shunzo Onishi
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Fujitani, N., Kawaguchi, N., Toda, S. et al. Immunocytochemical detection of enhanced expression of c-myc protein in the heart of cardiomyopathic hamster. Mol Cell Biochem 169, 73–78 (1997). https://doi.org/10.1023/A:1006829700747

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