Skip to main content
SpringerLink
Log in

Influence of retinol deficiency and curcumin/turmeric feeding on tissue microsomal membrane lipid peroxidation and fatty acids in rats

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The effect of retinol deficiency and curcumin/turmeric on lipid peroxidation and fatty acid profile was studied in liver, kidney, spleen and brain microsomes of rats. Results revealed an increase in lipid peroxidation in retinol deficient liver by 32%, kidney 30%, spleen 24% and brain 43% compared to the controls. Feeding 0.1% curcumin or turmeric for three weeks in diet to retinol deficient rats reduced the lipid peroxidation respectively to 12.5 or 22.6%, in liver, 23.7 or 24.1% in kidney, 14.4 or 18.0% spleen and 16.0 or 31.4% in brain. Retinol deficiency lead to a reduction in the essential fatty acids. In liver C18:1 showed a reduction by 45.6%, C18:2 by 31.6% and C20:4 by 22.8%. In kidney C18:1 was reduced by 33.6%, 18:2 by 24.6% and 20:4 by 13.7%. In spleen and brain C18:1 showed a reduction by 10.2% and 33.9%, C18:2 by 37.9% and 12.1% and C20:4 by 15.7% and 35.3% respectively. Curcumin and turmeric fed group showed a significant increase in the abnormally reduced fatty acid levels. (Mol Cell Biochem 175: 43–48, 1997)

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Weber F: Biochemical mechanisms of vitamin-A action. Proc Nutr Soc 42: 31–40, 1983

    Google Scholar 

  2. Giguere V, Ong ES, Segui P, Evanes RM: Identification of receptor for the morphogen retinoic acid. Nature 330: 624–629, 1987

    Google Scholar 

  3. Chytil F, Ong DE: Intracellular vitamin-A binding proteins. Ann Rev Nutr 7: 321–335, 1987

    Google Scholar 

  4. West KP: Dietary vitamin-A deficiency: Effects on growth, infection and mortality. Food and Nutr Bull 13: 119–131, 1991

    Google Scholar 

  5. Mack JP, Lui NST, Roels OA, Anderson OR: The occurrence of vitamin-A in biological membranes. Biochim Biophys Acta 288: 203–219, 1972

    Google Scholar 

  6. Grolier P, Cisti A, Danbeze M, Narbonne JF: The influence of dietary Vitamin-A intake on microsomal membrane fluidity and lipid composition. Nutr Res 11: 567–574, 1991

    Google Scholar 

  7. Periqet B, Bailley A, Perquet A, Ghisolfi J, Thouvert JP: Evidence for two subcellular pools and different kinetic behaviour of retinyl palmitate in rat liver. Internat J Vit Nutr Res 55: 245–251, 1985

    Google Scholar 

  8. Halliwell, Guteridge JMC: Free radicals in Biology and Medicine. Clarendon Press. Oxford, UK 1989

    Google Scholar 

  9. Ohyashiki T, Karino T, Matsui K: Stimulation of Fe induced lipid peroxidation in phosphatidylcholine liposomes by aluminium ions at physiological pH. Biochim Biophys Acta 1170: 182–188, 1993

    Google Scholar 

  10. Yasni S, Yoshiie K, Oda H, Sugano M, Imaizumi K: Dietary Curcuma Xanthorrhiza Roxb increases mitogenic response of splenic lymphocytes in mice. J Nutr Sci Vitaminol 39: 345–354, 1993

    Google Scholar 

  11. Shantha K, Krishnakantha TP: Microsomal Na+K+ activated Adenosine Triphosphatase activity in Retinol Deficient Albino Rats. Nutr Rep Internat 36: 573–580, 1987

    Google Scholar 

  12. Kartha VNR, Krishnamurthy S: Antioxidant function of vitamin-A. Internat J Vit Nutr Res 47: 393–401, 1977

    Google Scholar 

  13. Coaccio M, Valenza M, Tesoriere L, Bongiorno A, Albiero B, Livrea MA: Vitamin-A inhibits Doxorubicin-induced membrane lipid peroxidation in rat tissues in vivo. Arch Biochem Biophys 302: 103–103, 1993

    Google Scholar 

  14. Kaul S, Krishnakanth TP: Effect of retinol deficiency and curcumin or turmeric feeding on brain Na+K+ ATPase activity. Mol Cell Biochem 137: 101–107, 1994

    Google Scholar 

  15. Yehuda G, Shamir YK: Effect of urea sodium and calcium on microsomal ATPase activity in different parts of the kidney. Biochem Biophys Acta 233: 133–136, 1971

    Google Scholar 

  16. Miller DM, Aust SD: Studies on ascorbate dependent iron catalysed lipid peroxidation. Arch Biochem Biophys 271: 113–119, 1989

    Google Scholar 

  17. Bligh EG, Dyer WJ: A rapid method for the lipid extraction and purification. Can J Biochem Physiol 37: 911–917, 1959

    Google Scholar 

  18. Kates M: In: Laboratory Techniques in Biochemistry and Molecular Biology 3: 279–283, 1972

    Google Scholar 

  19. Hartree EF: Determination of protein. A modification of Lowry method that gives a linear photometric response. Anal Biochem 48: 422–427, 1972

    Google Scholar 

  20. Snedecor GW, Cochran WG: Statistical Methods. The Iowa University Press, Ames, 1977, pp 100–110

    Google Scholar 

  21. Anderson OR, Roels OA, Pfister RM: Natural Dietary Retinol and alpha tocopherol erythrocyte structure in rats. Nature 213: 47–49, 1967

    Google Scholar 

  22. Haugl RPB, Louis SL, Mush K, Waldeck N, Williams MA: Liver plasma membranes from essential fatty acid deficient rats. Isolation, fatty acid composition and activities of 5′nucleotides, ATPase and adenylate cyclase. Biochim Biophys Acta 433: 150–163, 1976

    Google Scholar 

  23. Colin C, Norbonne JF, Migaud ML, Grolier P, Pelissier MA: Lipid peroxidation and BaP activation to mutagenic metabolites: In vivo influence of vitamin A, E and C and Glutathione in both dietary vitamin A sufficiency and deficiency. Mutation Res 246: 150–168, 1991

    Google Scholar 

  24. Paterson PG, Gorecki DKJ, Card RT: Vitamin E deficiency and erythrocyte deformability in the rat. J Nutr Biochem 5: 298–302, 1994

    Google Scholar 

  25. Chow CK: Vitamin E and oxidative stress. Free Radical Biol Med 11: 215–232, 1991

    Google Scholar 

  26. Sevanian A, Hochstein P: Mechanisms and consequences of lipid peroxidation in biological systems. Ann Rev Nutr 5: 365–390, 1985

    Google Scholar 

  27. Hayam I, Cogan U, Mokady S: Dietary oxidized oil enhances the activity of Na K ATPase and acetylcholine esterase and lowers the fluidity of rat erythrocyte membrane. J Nutr Biochem 4: 563–568, 1993

    Google Scholar 

  28. Stubbs CD: Essays in Biochemistry 19: 1–39, 1983

    Google Scholar 

  29. Liu J, Mori A: Monoamine metabolism provides an antioxidant defence in the brain against oxidant and free radical induced age. Arch Biochem Biophys 302: 118–127, 1993

    Google Scholar 

  30. Gershwin ME, Beach RS, Hurley LS: Nutrition and Immunity. Orlando, FL. Academic Press 1985, chap 4–12

    Google Scholar 

  31. Berlin E, Bhathena SJ, Judd JT, Clevidence BA, Peters RC: Human erythrocyte membrane fluidity and insulin binding are dependent of dietary fatty acids. J Nutr Biochem 5: 591–598, 1994

    Google Scholar 

  32. Hamm MW, Chan V, Wolf G: Liver microsomal membrane fluidity and lipid characteristics in vitamin A deficient rats. Biochem J 245: 907–910, 1987

    Google Scholar 

  33. Grolier P, Cisti A, Danbeze M, Narbonne JF: The influence of dietary vitamin A intake on microsomal membrane fluidity and lipid composition. Nutr Res 11: 567–574, 1991

    Google Scholar 

  34. Fujiyara YF, Umeda R, Igarashi O: Effect of sesamin and curcumin on desaturation and chain elongation on PUFA metabolism in primary cultured rat hepatocytes. J Nutr Vitaminol 38: 353–357, 1992

    Google Scholar 

  35. Kaul S, Krishnakanth TP: Tissue Na K activated adenosine triphosphate in retinol deficient albino rats. Intern J Vit Nutr Res 62: 285–290, 1992

    Google Scholar 

  36. Kaul S, Krishnakanth TP: Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats. Die Nahrung 37: 35–40, 1993

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, 570013, India

    Sangeeta Kaul & T.P. Krishnakantha

Authors
  1. Sangeeta Kaul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T.P. Krishnakantha
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaul, S., Krishnakantha, T. Influence of retinol deficiency and curcumin/turmeric feeding on tissue microsomal membrane lipid peroxidation and fatty acids in rats. Mol Cell Biochem 175, 43–48 (1997). https://doi.org/10.1023/A:1006829010327

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006829010327

Search

Navigation