Hypolipidemic action of curcumin, the active principle of turmeric (Curcuma longa) in streptozotocin induced diabetic rats


Streptozotocin-induced diabetic rats were maintained on 0.5% curcumin containing diet for 8 weeks. Blood cholesterol was lowered significantly by dietary curcumin in these diabetic animals. Cholesterol decrease was exclusively from LDL-VLDL fraction. Significant decrease in blood triglyceride and phospholipids was also brought about by dietary curcumin in diabetic rats. In a parallel study, wherein diabetic animals were maintained on a high cholesterol diet, the extents of hypercholesterolemia and phospholipidemia were still higher compared to those maintained on control diet. Curcumin exhibited lowering of cholesterol and phospholipid in these animals also. Liver cholesterol, triglyceride and phospholipid contents were elevated under diabetic conditions. Dietary curcumin showed a distinct tendency to counter these changes in lipid fractions of liver. This effect of curcumin was also seen in diabetic animals maintained on high cholesterol diet. Dietary curcumin also showed significant countering of renal cholesterol and triglycerides elevated in diabetic rats.

In order to understand the mechanism of hypocholesterolemic action of dietary curcumin, activities of hepatic cholesterol-7a-hydroxylase and HMG CoA reductase were measured. Hepatic cholesterol-7a-hydroxylase activity was markedly higher in curcumin fed diabetic animals suggesting a higher rate of cholesterol catabolism. (Mol Cell Biochem 166: 169-175, 1997)

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  1. 1.

    Schonfeld G: Diabetes, lipoproteins and atherosclerosis. 34: 45–50, 1985

    Google Scholar 

  2. 2.

    Steiner G: Diabetes, atherosclerosis and metabolic links. Drugs 36: 22–26, 1988

    Google Scholar 

  3. 3.

    Stamler J: Established relationship among diet, serum cholesterol and coronary heart diseases. Acta Med Scand 207: 433–446, 1980

    Google Scholar 

  4. 4.

    Haffner SM: Compositional changes in lipoproteins of subjects with NIDDM. J Lab Clin Med 118: 109–110, 1991

    Google Scholar 

  5. 5.

    American Diabetic Association. Nutritional recommendations and principles for individuals with diabetes mellitus. Diabetes Care 10: 126–132, 1986

    Google Scholar 

  6. 6.

    Nadkarni AK: Indian Materia Medica, Popular Prakashan Pvt. Ltd., Bombay, India. Vol. 1, 3rd edn. 1976: pp 63, 475, 835

    Google Scholar 

  7. 7.

    Carson JF: Chemistry and biological properties of onion and garlic. Food Rev Internat 3: 71–103, 1987

    Google Scholar 

  8. 8.

    Subbarao D, Chandrasekhara N, Satyanarayana MN, Srinivasan M: Effect of curcumin on serum and liver cholesterol levels in the rat. J Nutr 100: 1307–1315, 1970

    Google Scholar 

  9. 9.

    Sharma RD: An evaluation of hypocholesterolemic factor of fenugreek seeds. Nutr Rep Internat 33: 669–678, 1986

    Google Scholar 

  10. 10.

    Kawada T, Hagihara K, Iwai K: Effect of capsaicin on lipid metabolism in rats fed with high fat diet. J Nutr 116: 1272–1278, 1986

    Google Scholar 

  11. 11.

    Huggelt ASG, Nixon DN: Use of glucose oxidase, peroxidase and o-dianisidine in the determination of blood and urinary glucose. Lancet: 368–370, 1957

  12. 12.

    Folch J, Lees M, Sloane-Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509, 1957

    Google Scholar 

  13. 13.

    Searcy RL, Bergquist LM: A new color reaction for quantitation of serum cholesterol. Clin Chim Acta 5: 192–197, 1960

    Google Scholar 

  14. 14.

    Warnick GR, Albers JJ: A comprehensive evaluation of heparin-manganese procedure for estimating HDL-cholesterol. J Lipid Res 19: 65–76, 1978

    Google Scholar 

  15. 15.

    Fletcher MJ: A colorimetric method for estimating serum triglycerides. Clin Chim Acta 22: 393–397, 1968

    Google Scholar 

  16. 16.

    Charles J, Stewart M: Colorimetric determination of phospholipids with ammonium ferrothiocynate. Anal Biochem 104: 10–14, 1980

    Google Scholar 

  17. 17.

    Mitropoules KA, Balasubramaniam S: Cholesterol-7a-hydroxylase in rat liver microsomal preparations. Biochem J 128: 1–9, 1972

    Google Scholar 

  18. 18.

    Hassan AS, Hackley JJ, Jeffery EH: Role of glutathione in the regulation of hepatic cholesterol-7a-hydroxylase, the rate limiting enzyme of bile acid synthesis. Steroids 44: 373–380, 1984

    Google Scholar 

  19. 19.

    Srinivasan K, Sambaiah K: The effect of spices on cholesterol-7a-hydroxylase activity and on serum and hepatic cholesterol levels in the rat. Internat J Vit Nutr Res 61: 364–369, 1991

    Google Scholar 

  20. 20.

    Hulcher FH, Oleson WH: A simplified spectrophotometric assay for microsomal HMG-CoA reductase by measurement of CoA. Lipid Res 14: 625–631, 1973

    Google Scholar 

  21. 21.

    Hartree EF: A modification of the Lowry's method that gives a linear photometric response. Anal Biochem 48: 422–427, 1972

    Google Scholar 

  22. 22.

    Snedecor GW, Cochran WG: Statistical Methods-6th Edn., Iowa State University Press, Ames, USA: 1976, p 298

    Google Scholar 

  23. 23.

    Sosenko JM, Breskiw JL, Miettinen OS, Gabby KH: Hyperglycemia and plasma lipids — A prospective study of young insulin dependent diabetic patients. N Eng J Med 302: 650–654, 1980

    Google Scholar 

  24. 24.

    Sekhar N, Govindaswamy S: Effects of vanadate on plasma lipoprotein profiles in experimental diabetic rat. Biochem Internat 23: 935–940, 1991

    Google Scholar 

  25. 25.

    Suresh Babu P, Srinivasan K: Influence of dietary curcumin and cholesterol on the progression of experimentally induced diabetes in albino rat. Mol Cell Biochem 152: 13–21, 1995

    Google Scholar 

  26. 26.

    Maechler P, Wollheim CB, Bentzen CL, Niesor E: Role of the intestinal acyl-CoA: cholesterol acyltransferase activity in the hyperresponse of diabetic rats to dietary cholesterol. J Lip Res 33: 1475–1484, 1992

    Google Scholar 

  27. 27.

    Laakso M, Sarlund H, Mykkanen L: Insulin resistance is associated with lipid and lipoprotein abnormalities in subjects with varying degrees of glucose tolerance. Atherosclerosis 10: 223–231, 1990

    Google Scholar 

  28. 28.

    Taskinen MR: Quantitative and qualitative lipoprotein abnormalities in Diabetes 41: 12–17, 1992

    Google Scholar 

  29. 29.

    Akira E: The discovery and development of HMG CoA reductase inhibitors. J Lip Res 33: 1569–1582, 1992

    Google Scholar 

  30. 30.

    Grundy SM, Bilheimer DW: Inhibition of 3-HMG CoA reductase by mevinolin in familial hypercholesterolemia-heterozygotes: Effect of cholesterol balance. Proc Natl Acad Sci 81: 2538–2542, 1984

    Google Scholar 

  31. 31.

    Garg A, Grundy SM: Lovastatin for lowering cholesterol levels in NIDDM. New Eng J Med 318: 81–86, 1988

    Google Scholar 

  32. 32.

    Hommel E, Andersen P, Gall MA et al.: Plasma lipoprotein and renal function during simvastatin treatment in diabetic nephropathy. Diabetologia 35: 447–451, 1992

    Google Scholar 

  33. 33.

    Mayes PA: Cholesterol synthesis, transport and excretion. In Harpers Biochemistry, 22nd Edition, 1990, pp 249–260

  34. 34.

    Fears R: Cholesterol-7a-hydroxylase In: R Fears and JR Sabine (eds). CRC Press, Boca Raton, 1986, pp 116–132

    Google Scholar 

  35. 35.

    Ganesh Bhat B, Srinivasan MR, Chandrasckhara N: Influence of curcumin and capsaicin on the composition and secretion of bile in rats. J Fd Sci Technol 21: 225–227, 1984

    Google Scholar 

  36. 36.

    Danielsson H, Sjovall J: Bile acid metabolism. Ann Rev Biochem 44: 233–253, 1975

    Google Scholar 

  37. 37.

    Gustafsson BE, Angelin B, Erinarsson K, Gustafsson JA: Influence of cholestyramine on synthesis of cholesterol and bile acids in germfree rats. J Lipid Res 19: 972–977, 1978

    Google Scholar 

  38. 38.

    Shepherd J, Packard CJ, Bicken S et al.: Cholestyramine promotes receptor mediated LDL catabolism. New Eng J Med 302: 1219–1222, 1980

    Google Scholar 

  39. 39.

    Kovanen PT, Bitheimer DW, Goldstein JL et al.: Regulatory role of hepatic LDL receptors in vivo in dogs. Proc Natl Acad Sci 78: 1194–1198, 1981

    Google Scholar 

  40. 40.

    Reaven EP, Reaven GM: Mechanisms for development of diabetic hypertriglyceridemia in streotozotocin treated rats. J Clin Invest 54: 1167–1178, 1974

    Google Scholar 

  41. 41.

    Vantol A: Hypertriglyceridemia in diabetic rat, defective removal of serum VLDL. Atherosclerosis, 26: 117–128, 1977

    Google Scholar 

  42. 42.

    Begadade JD, Porte D Jr, Biorman EL: Acute insulin withdrawal and the regulation of plasma triglyceride removal in diabetic subjects. Diabetes 17: 127–132, 1968

    Google Scholar 

  43. 43.

    Srinivasan MR, Satyanarayana MN: Influence of capsaicin, curcumin and ferulic acid in rats fed high fat fed diets. J Biosci 12: 143–152, 1987

    Google Scholar 

  44. 44.

    Rajalingam R, Srinivasan N, Govindarajulu P: Effects of alloxan induced diabetes on lipid profiles in renal cortex and medulla of mature albino rats. Indian J Exp Biol 31: 577–579, 1993

    Google Scholar 

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Babu, P.S., Srinivasan, K. Hypolipidemic action of curcumin, the active principle of turmeric (Curcuma longa) in streptozotocin induced diabetic rats. Mol Cell Biochem 166, 169–175 (1997). https://doi.org/10.1023/A:1006819605211

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  • curcumin
  • diabetes mellitus
  • cholesterol metabolism
  • hypolipidemic action