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Interactions of protein kinase CK2 subunits

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Abstract

Several approaches have been used to study the interactions of the subunits of protein kinase CK2. The inactive mutant of CK2α that has Asp 156 mutated to Ala (CK2αA156) is able to bind the CK2β subunit and to compete effectively in this binding with wild-type subunits α and α′. The interaction between CK2αA156 and CK2β was also demonstrated by transfection of epitope-tagged cDNA constructs into COS-7 cells. Immunoprecipitation of epitope-tagged CK2αA156 coprecipitated the β subunit and vice-versa. The assay of the CK2 activity of the extracts obtained from cells transiently transfected with these different subunits yielded some surprising results: The CK2 specific phosphorylating activity of these cells transfected with the inactive CK2αA156 was considerably higher than the control cells transfected with vectors alone. Assays of the immunoprecipitated CK2αA156 expressed in these cells, however, demonstrated that the mutant was indeed inactive. It can be concluded that transfection of the inactive CK2αA156 affects the endogenous activity of CK2. Transfection experiments with CK2α and β subunits and CK2αA156 were also used to confirm the interaction of CK2 with the general CDK inhibitor p21WAF1/CIP1 co-transfected into these cells. Finally a search in the SwissProt databank for proteins with properties similar to those derived from the amino acid composition of CK2β indicated that CK2β is related to protein phosphatase 2A and to other phosphatases as well as to a subunit of some ion-transport ATPases.

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Authors and Affiliations

  1. Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Casilla, Santiago, Chile

    Iris Korn, Catherine C. Allende & Jorge E. Allende

  2. Laboratory of Cellular Development and Oncology, National Institutes of Dental Research, National Institute of Health, Bethesda, MD, USA

    Silvio Gutkind

  3. Department of Biochemistry, University of Cambridge, Cambridge, UK

    N. Srinivasan & Tom L. Blundell

Authors
  1. Iris Korn
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  2. Silvio Gutkind
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  3. N. Srinivasan
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  4. Tom L. Blundell
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  5. Catherine C. Allende
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  6. Jorge E. Allende
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Korn, I., Gutkind, S., Srinivasan, N. et al. Interactions of protein kinase CK2 subunits. Mol Cell Biochem 191, 75–83 (1999). https://doi.org/10.1023/A:1006818513560

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