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Searching interaction partners of protein kinase CK2β subunit by two-hybrid screening

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Abstract

To date, the intracellular regulation of protein kinase CK2 is unknown. However it was observed that the enzyme associates with several intracellular proteins and the formation of such molecular complexes may represent a mechanism for the control of CK2 activity. Using the Interaction Trap system in yeast, with the CK2β as a bait, we looked for CK2 partners. We present the identification of new potential partners of CK2β and it is hoped that their classification will help in understanding the physiological roles and the regulation of CK2 in the cell.

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Authors and Affiliations

  1. Biochemische Zellphysiologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Swen Grein & Walter Grein

  2. Laboratoire de Biochimie des Régulations Cellulaires Endocrines, INSERM U244, DBMS, CEA, Grenoble, France

    Karine Raymond, Claude Cochet, Edmond M. Chambaz & Odile Filhol

Authors
  1. Swen Grein
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  2. Karine Raymond
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  3. Claude Cochet
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  4. Walter Grein
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  5. Edmond M. Chambaz
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  6. Odile Filhol
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Grein, S., Raymond, K., Cochet, C. et al. Searching interaction partners of protein kinase CK2β subunit by two-hybrid screening. Mol Cell Biochem 191, 105–109 (1999). https://doi.org/10.1023/A:1006814428582

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