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Choline derived from the phosphatidylcholine specific phospholipase D is not directly available for the CDP choline pathway in phorbol ester- treated C3H10T1/2 C18 fibroblasts

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Abstract

We have shown that 12-O-tetradecanoylphorbol 13-acetate (TPA) increases protein kinase C (PKC)-mediated choline transport, incorporation of choline into phosphatidylcholine (PtdCho) and PtdCho degradation by phospholipase D (PLD) in C3H10T1/2 Cl 8 cells. Dual prelabeling experiment using [3H]/[14C]choline indicated that intracellular choline generated from the PLD reaction was not directly recycled to PtdCho synthesis within the cell, and that a large fraction of the choline was transported out of the TPA-treated cells. In contrast, medium derived choline was preferably channeled to PtdCho synthesis. These results indicate that in TPA-treated cells, the choline derived from the PKC-mediated increased PLD activity and the choline newly taken up by the cell behave as two distinctly different metabolic pools.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

    Vidar A.T. Thorsen, Ove Bruland, Johan R. Lillehaug & Holm Holmsen

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  1. Vidar A.T. Thorsen
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  2. Ove Bruland
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  3. Johan R. Lillehaug
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  4. Holm Holmsen
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Thorsen, V.A., Bruland, O., Lillehaug, J.R. et al. Choline derived from the phosphatidylcholine specific phospholipase D is not directly available for the CDP choline pathway in phorbol ester- treated C3H10T1/2 C18 fibroblasts. Mol Cell Biochem 187, 147–154 (1998). https://doi.org/10.1023/A:1006813524791

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