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Proteolysis activated protein kinase in Dictyostelium discoideum

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Abstract

In the search for MBP phosphorylating activities in Dictyostelium discoideum, we have found a proteolysis-activated protein kinase. This activity which is distributed between the soluble and the particulate fractions of the cell, uses MBP and histone as substrate and has a molecular mass of 140 kDa as detected in an ’in situ' assay.

This protein kinase has several features shared by the protein kinase C family, such as substrate specificity and sensitivity to proteolysis, but its molecular mass is much larger than that described for the known protein kinase C isoforms.

To better characterize this activity we have studied its sensitivity to several protein kinase C inhibitors and activators. This protein kinase is activated neither by phorbol ester nor by phosphatidylserine or Ca2+. The activity is inhibited by staurosporine and PKC ζ pseudosubstrate, but is not affected by the specific protein kinase C inhibitor bisindolylmaleimide.

These data lead us to propose that proteolytically activated Dictyostelium protein kinase belongs to the recently described protein kinase C-related family.

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  1. Departamento de Bioquímica Universidad Autónoma de Madrid, Instituto de Investigaciones Biomédicas, Madrid, España

    Alicia Nuñez & Margarita Fernández-Renart

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  1. Alicia Nuñez
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  2. Margarita Fernández-Renart
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Nuñez, A., Fernández-Renart, M. Proteolysis activated protein kinase in Dictyostelium discoideum. Mol Cell Biochem 175, 177–185 (1997). https://doi.org/10.1023/A:1006809202539

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