Abstract
The efficacy of granulocyte–macrophage colony-stimulating factor (GM-CSF) to enhance the primary immune response to hepatitis B vaccine was studied in healthy elderly with young volunteers included as controls in this double-blind, placebo-controlled trial of GM-CSF as an immune adjuvant. Na¨ıve T-helper cells (CD4+CD45RA+) were determined at baseline. Forty-five healthy elderly (average age, 74 years) and 37 healthy young controls (average age, 28 years) were randomized. Hepatitis B vaccine was administered at 0, 1, and 6 months. GM-CSF as a single injection of either 80 μg or 250 μg with the first and second doses of hepatitis B vaccine. In this trial GM-CSF did not enhance antibody responses. However, the antibody responses were dramatically different between these two groups: 35/35 young developed a protective titer versus 19/45 elderly (P < 0.0001). In addition, the mean logarithm of anti-hepatitis B antibody level in the 35 young who completed the study was 3.17 (log mIU/ml) but only 2.21 in the 19 elderly responders (P < 0.0001). Na¨ıve T-helper cells differed significantly between the two groups: the mean percentage of CD4+CD45RA+ T cells was 47.9% versus 35.0% (P < 0.0001) in the young and elderly volunteers respectively. Na¨ıve T cells also differed significantly between elderly who did or did not respond to HBV (39.9% vs. 31.7%, P = 0.039). Using linear regression, age, and percent na¨ıve, CD4 T cells were determined to significantly influence the anti-hepatitis B antibody response, but sex and dose of GM-CSF did not. For a two-parameter model: logarithm of antibody titer = (−0.038 × age in years) + (0.031 × % na¨ıve CD4T cells) + 2.68; adjusted r2 = 0.605 and P < 0.0001. However, age had a larger effect than na¨ıve CD4 T cells, i.e., in comparing young and elderly groups the log antibody titer decreased by 1.73 due to the increase in age but only 0.40 due to the decrease in na¨ıve CD4 T cells. Thus, there was a large effect of age that could not be explained by the quantitative change in the na¨ıve T-helper cells.
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REFERENCES
Kishimoto S, Tomino S, Mitsuya H, Fujiwara H, Tsuda H: Age-related decline in the in vitro and in vivo syntheses of anti-tetanus toxoid antibody in humans. J Immunol 125:2347–2352, 1980
Cook JM, Gualde N, Hessel L, et al.: Alterations in the human immune response to the hepatitis B vaccine among the elderly. Cell Immunol 109:89–96, 1987
Mastroeni I, Vescia N, Pompa MG, Cattaruzza MS, Marini GP, Fara GM: Immune response of the elderly to rabies vaccines. Vaccine 12:518–520, 1994
de Rave S, Heijtink RA, Bakker-Bendik M, Boot J, Schalm SW: Immunogenicity of standard and low dose vaccination using yeast-derived recombinant hepatitis B surface antigen in elderly volunteers. Vaccine 12:532–534, 1994
De Paoli P, Battistin S, Santini GF: Age-related changes in human lymphocyte subsets: Progressive reduction in the CD4CD45R (suppressor inducer) population. Clin Immunol Immunopathol 48:290–296, 1988
Jackola DR, Ruger JK, Miller RA: Age-associated changes in human T cell phenotype and function. Aging 6:25–34, 1994
Utsuyama M, Hiokawa K, Kurashima C, et al.: Differential age-change in the numbers of CD41CD45RA1 and CD41CD291 T cell subsets in human peripheral blood. Mech Aging Dev 86:57–68, 1992
Cossarizza A, Ortolani C, Paganelli R, et al.: Mech Aging Dev 63:57–68, 1992
Lin R, Tarr PE, Jones TC: Present status of the use of cytokines as adjuvants with vaccines to protect against infectious diseases. Clin Infect Dis 21:1439–1449, 1995
Tarr PE, Lin R, Mueller EA, Kovarik JM, Guillaume M, Jones TC: Evaluation of tolerability and antibody response after recombinant human granulocyte-macrophage colony-stimulating factor (rhGMCSF) and a single dose of recombinant hepatitis B vaccine. Vaccine 14:1199–1204, 1996
Disis ML, Bernhard H, Shiota FM, et al.: Granulocyte-macrophage colony-stimulating factor: An effective adjuvant for protein and peptide-based vaccines. Blood 88:202–210, 1996
Fischer HG, Opel B, Reske K, Reske-Kunz AB: Granulocyte-macrophage colony-stimulating factor-cultured bone marrowderived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli. Eur J Immunol 18:1151–1158, 1988
Larsen CP, Ritchie SC, Hendrix R, et al.: Regulation of immunostimulatory function and costimulatory molecule (B7–1 and B7–2) expression on murine dendritic cells. J Immunol 152:5208–5219, 1994
Markowicz S, Engleman EG: Granulocyte-macrophage colonystimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J Clin Invest 85:955–961, 1990
Steinman RM: The dendritic cell system and its role in immunogenicity. Annu Rev Immunol 9:271–296, 1991
Phillips N, Jacobs S, Stoller R, Earle M, Przepiorka D, Shadduck RK: Effect of recombinant human granulocyte-macrophage colony-stimulating factor on myelopoiesis in patients with refractory metastatic carcinoma. Blood 74:26–34, 1989
Schulz G, Frisch J, Greifenberg B, Nicolay U, Oster W: New therapeutic modalities for the clinical use of rhGM-CSF in patients with malignancies. Am J Clin Oncol 14 Suppl 1:S19–26, 1991
Bodey GP, Anaissie E, Gutterman J, Vadhan-Raj S: Role of granulocyte-macrophage colony-stimulating factor as adjuvant treatment in neutropenic patients with bacterial and fungal infection. Eur J Clin Microbiol Infect Dis 13 Suppl 2:S18–22, 1994
Mamounas EP, Anderson S, Wickerham DL, et al.: The efficacy of recombinant human granulocyte colony-stimulating factor and recombinant human granulocyte macrophage colony-stimulating factor in permitting the administration of higher doses of cyclophosphamide in a doxorubicin-cyclophosphamide combination. An NSABP pilot study in patients with metastatic or high-risk primary breast cancer. National Surgical Adjuvant Breast and Bowel Project. Am J Clin Oncol 17:374–381, discussion 382, 1994
Hassan HT, Zander AR: Thrombocytopenia after high-dose chemotherapy and autologous stem cell transplantation: An unresolved problem and possible approaches to resolve it. Journal of Hematotherapy 5:407–414, 1996
Hebert JC, O'Reilly M: Granulocyte-macrophage colonystimulating factor (GM-CSF) enhances pulmonary defenses against pneumococcal infections after splenectomy. J Trauma Injury Infect Crit Care 41:663–666, 1996
Bodey GP, Anaissie E, Gutterman J, Vadhan-Raj S: Role of granulocyte-macrophage colony-stimulating factor as adjuvant therapy for fungal infection in patients with cancer. Clin Infect Dis 17:705–707, 1993
Hasan MS, Agosti JM, Reynolds KK, Tanzman E, Treanor JJ, Evans TG: Granulocyte-macrophage colony-stimulating factor as an adjuvant for hepatitis B vaccination in healthy adults. J Infect Dis 180:2023–2026, 1999
Hess G, Kreiter F, Kosters W, Deusch K: The effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on hepatitis B vaccination in haemodialysis patients. J Viral Hepatitis 3:149–153, 1996
Samanci A, Yi Q, Fagerberg J, Strigard K, Smith G, Ruden U, Wahren B, Mellstedt H: Pharmacological administration of granulocyte/ macrophage-colony-stimulating factor is of significant importance for the induction of a strong humoral and cellular response in patients immunized with recombinant carcinoembryonic antigen. Cancer Immunol Immunother 47(3):131–142, 1998
Weng NP, Levine BL, June CH, Hodes RJ: Human naive and memory T lymphocytes differ in telomeric length and replicative potential. Proc Nat Acad Sci USA 92:11091–11094, 1995
Kong FK, Chen CL, Six A, Hockett RD, Cooper MD: T cell receptor gene deletion circles identify recent thymic emigrants in the peripheral T cell pool. Proc Nat Acad Sci USA 96:1536–1540, 1999
Spencer NF, Poynter ME, Im SY, Daynes RA: Constitutive activation of NF-kappa B in an animal model of aging. Int Immunol 9:1581–1588, 1997
Sunderkotter C, Kalden H, Luger TA: Aging and the skin immune system. Arch Dermatol 133:1256–1262, 1997
Steger MM, Maczek C, Grubeck-Loebenstein B: Morphologically and functionally intact dendritic cells can be derived from the peripheral blood of aged individuals. Clin Exp Immunol 105:544–550, 1996
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Looney, R.J., Hasan, M.S., Coffin, D. et al. Hepatitis B Immunization of Healthy Elderly Adults: Relationship between Na¨ıve CD4+ T Cells and Primary Immune Response and Evaluation of GM-CSF as an Adjuvant. J Clin Immunol 21, 30–36 (2001). https://doi.org/10.1023/A:1006736931381
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DOI: https://doi.org/10.1023/A:1006736931381