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Simplified clonal multiplication of mulberry using liquid shake culture

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Abstract

Shoot apex and axillary bud explants of mulberry (Morus indica) were cultured in liquid Murashige and Skoog medium containing N-(2-chloro-4-pyridyl)-N′-phenylurea (a urea-type cytokinin) at 0.5–2 mg l-1. The cultures were maintained at 120 rpm up to 6 weeks. During the first 4 weeks, shoot induction and shoot elongation were observed, followed by adequate rooting during the latter 2 weeks in both explants. A single-step liquid medium which induces consecutive development of shoots and roots in a same medium is useful for effective multiplication of mulberry plantlets with reduced labor and cost.

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Author notes

  1. Prakash K. Tewary

    Present address: Central Sericultural Research Institute, Mysore, 570 008, India

  2. Seibi Oka

    Present address: Plant Biotechnology Institute, Ibaraki Agricultural Center, Ago Iwama, Ibaraki, 319-0292, Japan requests for offprints. E-mail

Authors and Affiliations

  1. National Institute of Agrobiological Resources, Tsukuba, Ibaraki, 305-8602, Japan

    Prakash K. Tewary & Seibi Oka

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  1. Prakash K. Tewary
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  2. Seibi Oka
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Tewary, P.K., Oka, S. Simplified clonal multiplication of mulberry using liquid shake culture. Plant Cell, Tissue and Organ Culture 59, 223–226 (1999). https://doi.org/10.1023/A:1006496323743

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  • DOI: https://doi.org/10.1023/A:1006496323743

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