Abstract
Through immature seed culture and subsequent embryo culture, aneuploid plants were derived from various crosses among 184 different triploid hybrid grape vines. In self-pollinations of the 184 vines, 0 to 1.6% of flowers produced immature seeds. In 16 reciprocal crosses between diploid and triploid and between tetraploid and triploid grapes, 0 to 23.0% of flowers produced immature seeds. The immature seeds excised 30–50 days after pollination were cultured for three months on Nitsch and Nitsch medium supplemented with L-glutamine, L-serine, L-cysteine and casein hydrolysate. Embryos developed within the cultured immature seeds were subcultured onto germination medium consisting of MS medium with 1 μM BA. Thirty-four of 137 embryos from 458 immature seeds germinated. Five of the 34 embryos grew normally. The five recovered plants were aneuploids with chromosome numbers from 51 to 59. The rates of embryo and plant recovery were different in different crosses with triploid grapes.
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References
Belling J & Blakeslee AF (1924) The configurations and sizes of the chromosomes in the trivalents of 25-chromosome Daturas. Proc. Natl. Acad. Sci. USA 10: 116–120
Burnham CR (1962) Discussion in Cytogenetics. Burgress, Minneapolis, MN
Cain DW, Emershad RL & Tarailo RE (1983) In ovulo embryo culture and seedling development of seeded and seedless grapes (Vitis vinifera L.). Vitis 22: 9–14
Dyck PL & Rajhathy T (1965) A desynaptic mutant in Avena strigosa. Can. J. Genet. Cytol. 7: 418–421
Emershad RL & Ramming DW (1984) In ovulo embryo culture of vitis vinifera L. cv. 'Thompson seedless'. Amer. J. Bot. 71: 873–877
Emershad RL, Ramming DW & Serpe MD (1989) In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera. Amer. J. Bot. 76: 397–402
Goodspeed TH & Avery P (1939) Trisomic and other types in Nicotiana sylvestris. J. Genet. 38: 381–458
Gray DJ, Fisher LC & Mortensen JA (1987) Comparison of methodologies for in ovulo embryo rescue of seedless grapes. HortScience 22: 1334–1335
Gray DJ, Mortensen JA & Benton CM (1990) Ovule culture to obtain progeny from hybrid seedless bunch grapes. J. Amer. Soc. Hort. Sci. 115: 1019–1024
Kamanoi M & Jenkins BC (1962) Trisomics in common rye, Secale cereale L. Seiken Ziho 13: 118–123 (in Japanese with English summary)
Khush GS, Singh RJ, Sur SC & Librojo AL (1984) Primary trisomics of rice; origin, morphology, cytology and use in linkage mapping. Genetics 107: 141–163
Koller PC (1938) Asynapsis in Pisum sativum. J. Genet. 36: 275–306
Kuspira J, Bhambhani RN, Sadasivaiah RS & Hayden D (1986) Genetic and cytogenetic analyses of the A genome of Triticum monococcum. III. Cytology, breeding behavior, fertility, and morphology of autotriploid. Can. J. Genet. Cytol. 28: 867–887
Martin A (1978) Aneuploidy in Vicia faba L. J. Hered. 69: 421–423
Murashige T & Skoog FM (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant. 15: 473–497
Nitsch JP & Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87
Ramage RT (1960) Trisomics from interchange heterozygotes in barley. Agron. J. 52: 156–159
Satina S, Blakeslee AF & Avery AG (1938) Chromosome behavior in triploid Datura. III. The seed. Am. J. Bot. 25: 595–602
Spiegel-Roy P, Sahar N, Baron J & Lavi U (1985) In vitro culture and plant formation from grape cultivars with abortive ovules and seeds. J. Amer. Soc. Hort. Sci. 110: 109–112
Stewart JM & Hsu CL (1977) In ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137: 113–117
Tabushi J (1958) Trisomics of spinach. Seiken Ziho 9: 49–57 (in Japanese with English summary)
Tsuchiya T (1964) Chromosome aberrations and their use in genetics and breeding in barley-trisomics and aneuploids. Proc. 1st Intern. Barley Genet. Symp. 1: 116–150
Yamane H, Kurihara A & Tanaka R (1978) Studies on polyploidy breeding in grapes. I. Chromosome numbers of large-berried grape varieties grown in Japan. Bull. Fruit Tree Res. Stn. E 2: 1–8 (in Japanese with English summary)
Zhuang DH, Kitajima A, Ishida M & Sobajima Y (1990) Chromosome numbers of Diospyros kaki cultivars. J. Japan. Soc. Hort. Sci. 59: 289–297 (in Japanese with English summary)
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Park, S., Hiramatsu, M. & Wakana, A. Aneuploid plants derived from crosses with triploid grapes through immature seed culture and subsequent embryo culture. Plant Cell, Tissue and Organ Culture 59, 125–133 (1999). https://doi.org/10.1023/A:1006477422833
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DOI: https://doi.org/10.1023/A:1006477422833