Abstract
The effect of several media components on the germination percentage of ovules in intraspecific T. gesneriana L. crosses was studied by using two embryo rescue techniques, viz. ovary-slice culture followed by ovule culture and direct ovule culture. The addition of 9% sucrose to medium for ovary-slice culture, started at 3 or at 5 weeks after pollination (WAP), significantly improved the germination percentage as compared to 5% sucrose. The germination percentage did not differ between both sucrose concentrations (3% and 5%) used in ovule culture started 4 weeks later with ovules excised from the ovary-slices (at 9 WAP). Similar germination percentages were obtained with media containing the full or half of the concentrations of micronutrients and macronutrients of the MS-medium during ovary-slice culture and ovule culture. For direct ovule culture, started at 4, at 6, and at 8 WAP, the germination percentages did not differ between ovules cultured on media with 3%, 6% or 9% sucrose. The addition of the cytokinin BAP (0.01 or 0.1 mg l-1) had no effect on the germination percentage. The use of liquid-shaken culture resulted in germination percentages which were similar to those on agar-solidified media. Analysis of the carbohydrate concentration of the media revealed that, in both media for ovary-slice culture and for ovule culture, ultimately all sucrose is converted into glucose and fructose. The total concentration of carbohydrates decreased with 19%–48% in the media for ovary-slice culture, whereas the total concentration of carbohydrates did not decrease remarkably in media for ovule culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buitendijk JH, Pinsonneaux N, van Donk AC, Ramanna MS& van Lammeren AAM (1995) Embryo rescue by half-ovule culture for the production of interspecific hybrids in Alstroemeria. Sci. Hortic. 64: 65–75
Campenot MK, Zhang G, Cutler AJ& Cass DD (1992) Zea mays embryo sacs in culture. I. Plant regeneration from 1 day after pollination embryos. Am. J. Bot. 79: 1368–1373
Cohen D, Ladizinsky G, Ziv M& Muehlbauer FJ (1984) Rescue of interspecific Lens hybrids by means of embryo culture. Plant Cell Tiss. Org. Cult. 3: 343–347
Custers JBM, Eikelboom E, Bergervoet JHW& van Eijk JP (1992) In ovulo embryo culture of tulip (Tulipa L.); effects on culture conditions on seedling and bulbet formation. Sci. Hortic. 51: 111–122
Custers JBM, Eikelboom W, Bergervoet JHW& van Eijk JP (1995) Embryo-rescue in the genus Tulipa L.; successful direct transfer of T. kaufmanniana Regel germplasm into T. gesneriana L. Euphytica 82: 253–261
Gudin S (1994) Embryo rescue in Rosa hybrida L. Euphytica 72: 205–212
Kobayashi RS, Sinden SL& Bouwkamp JC (1993) Ovule culture of sweet potato (Ipomoea batatas) and closely related species. Plant Cell Tiss. Org. Cult. 32: 77–82
Lipavska H& Vreugdenhil D (1996) Uptake of mannitol from the media by in vitro grown plants. Plant Cell Tiss. Org. Cult. 45: 103–107
McCullagh P& Nelder JA (1989) Generalized Linear Models. Second edition, Chapman&Hall, London: 151
Murashige T& Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Neal CA& Topoleski LD (1983) Effects of the basal medium on growth of immature tomato embryos in vitro. J. Am. Soc. Hort. Sci. 108: 434–438
Payne RW, Ainsley AE, Bicknell KE& Franklin MF (1993) Genstat 5 Release 3 Reference Manual. Clarendon Press, Oxford, Great Britain: 413–417
Raghavan V& Torrey JG (1963) Growth and morphogenesis of globular and older embryos of Capsella in culture. Am. J. Bot. 50: 540–551
Savka MA, Jokela JJ, Skirvin RM& Dawson JO (1985) Culture of ovules containing immature embryos of eastern cottonwood in vitro. In: Dawson JO& Majerus UA (ed) Proc. Fifth Central Hardwood Conf. Dept of Forestry, Univ. of Illinois: 234–238
Schmitz U& Lörz H (1990) Nutrient uptake in suspension cultures of Gramineae. I. Development of an assay system. P. Sci. 66: 87–94
Sharma DR, Kaur R& Kumar K (1996) Embryo rescue in plants – a review. Euphytica 89: 325–337
Tremblay L& Tremblay FM (1995) Maturation of black spruce somatic embryos: Sucrose hydrolysis and resulting osmotic pressure of the medium. Plant Cell Tiss. Org. Cult. 42: 39–46
Van Creij MGM, Kerckhoffs DMFJ& van Tuyl JM (1999) The effect of ovule age on ovary-slice culture and ovule culture in intraspecific and interspecific crosses with Tulipa gesneriana L. Euphytica 108: 21–28
Van Eijk JP, van Raamsdonk LWD, Eikelboom W& Bino RJ (1991) Interspecific crosses between Tulipa gesneriana cultivars and wild Tulipa species: a survey. Sex. Pl. Reprod. 4: 1–5
Van Raamsdonk LWD, van Eijk JP& Eikelboom W (1995) Crossability analysis in subgenus Tulipa of the genus Tulipa L. Bot. J. Linn. Soc. 117: 147–158
Williams EG, Maheswaran G& Hutchinson JF (1987) Embryo and ovule culture in crop improvement. Plant Breed. Rev. 5: 181–236
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Creij, M., Kerckhoffs, D., De Bruijn, S. et al. The effect of medium composition on ovary-slice culture and ovule culture in intraspecific Tulipa gesneriana crosses. Plant Cell, Tissue and Organ Culture 60, 61–67 (2000). https://doi.org/10.1023/A:1006476621910
Issue Date:
DOI: https://doi.org/10.1023/A:1006476621910